Cell Culture Method
Cell Culture Method - Related Videos
01:33
Cell Culture Methods for New Approach Methodologies
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2026
Mammalian and other animal-derived cell culture systems are widely used in biomedical research as New Approach Methodologies (NAMs) that reduce reliance on animal testing while enabling controlled investigation of cellular responses. These in vitro systems provide reproducible platforms for studying disease mechanisms, drug activity, toxicity, and neuronal function under defined laboratory conditions. Establishment and Maintenance of Cell Cultures To establish a culture, cells are seeded into...
09:10
A Method for Culturing Embryonic C. elegans Cells
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Cited by 13 •
2013
We describe here a revised protocol for large-scale culture of embryonic C. elegans cells. Embryonic C. elegans cells cultured in vitro using this method, appear to differentiate and recapitulate the expression of genes in a cell specific manner. Techniques that require direct access to the cells or isolation of specific cell types from the other tissues can be applied on C. elegans cultured cells.
04:41
Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts
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2023
This video explains the 3D magnetic bioprinting of co-cultured pancreatic cancer cells and fibroblasts. Pancreatic cancer cells and activated pancreatic fibroblast cells are co-cultured and magnetized using a biocompatible nanoparticle assembly, to generate spheroids, under the influence of magnetic forces.
03:27
2D Co-culture of Leukemic Cells with BMSCs: A Method to Obtain Subpopulations of Leukemic Cells
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2023
This video describes the technique for long term 2D co-culture of leukemia cells with bone marrow stromal cells to study their interaction with the bone marrow microenvironment. The 2D coculture model utilizes the acute lymphoblastic leukemia cells and the bone marrow stromal or osteoblasts that influence tumor cell phenotype. This study will support investigation of the mechanisms that underlie bone marrow supported survival of leukemic cells during chemotherapy exposure.
04:01
Spheroid Generation from Cell Lines: A Three-Dimensional (3D) Cell Culture Method
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2023
Spheroids are a type of 3D cell culture model used to more accurately represent a cell’s environment compared to 2D cell culture monolayer models. In the example protocol, we will see scientists generate spheroids from a colorectal cancer cell line and apply live-cell imaging to track the spheroids’ growth.
FAQs
These conditions help determine whether cells remain viable, proliferate, or respond appropriately to developmental signals. The culture surface or matrix provides physical support, while temperature and gas balance create the surrounding environment in which cellular processes occur. Because changing these conditions can alter growth or development, researchers control them when comparing cell behaviors across experiments.
Regular medium changes help sustain cells as they grow, while passaging prevents cultures from becoming overly crowded and allows continued expansion. Together, these steps maintain conditions suitable for experimental observation and manipulation. Consistent handling also makes it easier to compare developmental outcomes, such as differences in proliferation or differentiation, between experimental groups.
Researchers can introduce experimental signals to test how cells interpret genes, signaling pathways, or environmental factors. These inputs may shift developmental behavior, including whether cells continue proliferating, begin differentiating, or adopt a particular lineage. Comparing treated and controlled cultures helps connect a specific signal with changes in cell fate and development.
The model should match the developmental process being examined. Cultured cells can support studies of proliferation and differentiation, whereas tissues and stem-cell-derived models can provide information about lineage specification and tissue organization. Selecting among these systems allows researchers to investigate developmental behavior at the level most relevant to the question.
Cells are placed in sterile vessels with nutrient medium and provided with suitable temperature, gas balance, and surface or matrix support. Researchers then change the medium regularly and passage the culture as needed to sustain growth. Once the system is stable, experimental signals can be applied and resulting changes in development can be examined.
It is useful when researchers need to observe these processes directly while controlling the surrounding conditions. Cultured models allow experimental signals, genes, signaling pathways, and environmental factors to be tested individually or in combination. The resulting changes can reveal how cells progress through developmental behaviors that may be difficult to isolate within an organism.
Tissues and stem-cell-derived models can be used to examine how cells organize as they develop, while controlled cultures support tests of factors associated with normal development or disease. By comparing developmental outcomes under different signals or environmental conditions, researchers can investigate disrupted proliferation, differentiation, lineage specification, or tissue organization in a focused experimental setting.