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A Method for Culturing Embryonic C. elegans Cells
JoVE Journal
Biology
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JoVE Journal Biology
A Method for Culturing Embryonic C. elegans Cells
DOI:

09:10 min

September 21, 2013

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Chapters

  • 00:05Title
  • 01:01Egg Isolation
  • 04:15Embryonic Cell Dissociation and Culturing
  • 06:00Cultured Embryonic C. elegans Cell Results
  • 08:47Conclusion

Summary

Automatic Translation

We describe here a revised protocol for large-scale culture of embryonic C. elegans cells. Embryonic C. elegans cells cultured in vitro using this method, appear to differentiate and recapitulate the expression of genes in a cell specific manner. Techniques that require direct access to the cells or isolation of specific cell types from the other tissues can be applied on C. elegans cultured cells.

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