Hand Pollination

Hand pollination is a controlled technique in which pollen is manually transferred from a flower’s anther to the stigma of the same or another flower, allowing researchers to manage plant reproduction. The process typically involves selecting receptive flowers, collecting pollen, and applying it directly to the stigma while preventing unwanted pollen transfer through isolation or protective covering. In developmental biology, hand pollination enables controlled crosses for studying fertilization, embryo and seed formation, inheritance, and reproductive timing. It also supports plant breeding and experimental analyses of how genetic contributions and environmental conditions influence early development.

Hand Pollination - Related Videos

Education

JoVE Core - Biology

Pollination and Flower Structure

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2020

Flowers are the reproductive, seed-producing structures of angiosperms. Typically, flowers consist of sepals, petals, stamens, and carpels. Sepals and petals are the vegetative flower organs. Stamens and carpels are the reproductive organs. Flowers must be pollinated to produce seeds. In angiosperms, pollination is the transfer of pollen from the anther of the stamen (the male structure) to the stigma of the carpel (the female structure). Flowers may be self-pollinated or cross-pollinated.

Research

JoVE Journal - Developmental Biology

Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping

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Cited by 6 •

2020

A methodology for the determination of pollination requirements in Japanese plum-type hybrids is described, which combines field- and laboratory-pollinations and observations of pollen tubes under the fluorescence microscopy with the identification of S-genotypes by PCR and the monitoring of flowering for the selection of pollinizers.

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

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Cited by 3 •

2020

We present a methodology to establish the pollination requirements of apricot (Prunus armeniaca L.) cultivars combining the determination of self-(in)compatibility by fluorescence microscopy with the identification of the S-genotype by PCR analysis.

Field Experiments of Pollination Ecology: The Case of Lycoris sanguinea var. sanguinea

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Cited by 1 •

2016

To reveal the pollinator effectiveness of a given plant species, multiple methods of field experiments have been developed. This study demonstrates the basic methods of field experiments for pollination ecology using the case study of Lycoris sanguinea var. sanguinea and the novel pollination mechanism, breaking-bud pollination.

Education

JoVE Science Education - Psychology
Free Sample

The Rubber Hand Illusion

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2023

Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Reaching for objects, walking without hitting obstacles, landing on a chair as you sit (instead of falling to the floor), these and all our physical actions depend on an ability to perceive our own bodies in space, to know where our limbs are relative to one another and relative to the rest of the world. One way that the human brain encodes this information is called proprioception, the brain relies on its own control and feedback...

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