Naive Primed Transition
Naive Primed Transition - Related Videos
06:16
Verbal Priming
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Human memory seems to work in two broad ways. Like modern computers, the human mind has explicit, or declarative, memory: ask a question, and a person gives the best answer they can. Input a query, and a computer program returns the contents of the relevant parts of its stored memory. Humans also have a second kind of memory system, one not really typical of computers, one that experimental psychologists call implicit. Implicit...
02:31
Phase Transitions
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2020
Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to occupy...
14:23
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
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Cited by 29 •
2012
We describe a method for generating regulatory, memory and naïve T cells from a single human blood donor. Polarized Tregs can be then compared to other subsets in a variety of genetic and functional applications with genetic homogeneity, including a suppression assay also detailed here.
12:59
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
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Cited by 33 •
2013
With effector functions distinct from other T cell subsets, Th17 cells have been centrally implicated in inflammatory autoimmunity. This in vitro Th17 differentiation protocol provides a means to determine whether naïve CD4+ T lymphocytes can differentiate into Th17 cells, and to further examine their role in autoimmunity and host response.
01:58
Cooperative Allosteric Transitions
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2020
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
FAQs
Extracellular signals alter how pluripotent cells interpret their developmental environment and respond to differentiation cues. As signaling conditions change, intracellular gene-regulatory networks also shift, reshaping the circuitry that maintains pluripotency. This interaction helps move cells toward a state with different identity, developmental responsiveness, and capacity for lineage priming, rather than producing a simple change in cell appearance.
The transition includes remodeling of intracellular gene-regulatory networks that control pluripotency and cell identity. These networks change alongside extracellular signaling, so the cells become responsive to a different set of developmental instructions. The resulting regulatory state supports lineage priming, meaning cells begin acquiring tendencies toward specialized fates while remaining within the broader pluripotent developmental framework.
Morphology and epigenetic state provide additional dimensions for characterizing the transition beyond pluripotency gene regulation alone. Changes in cell form accompany altered identity and developmental responsiveness, while epigenetic remodeling reflects changes in how cellular information is regulated. Considering these features together helps distinguish developmental progression from an isolated change in one molecular marker.
Researchers can examine embryonic stem cells and induced pluripotent stem cells as experimental systems for following this developmental shift. Studies can compare cells across changing signaling conditions while evaluating pluripotency circuitry, cell identity, lineage priming, morphology, and epigenetic state. This integrated approach connects molecular regulation with visible and functional changes relevant to early mammalian development.
These models provide an in vitro framework for investigating biology associated with the implantation-stage embryo, particularly the postimplantation developmental context. By examining how pluripotent cells change their regulatory state and responsiveness, researchers can study developmental events that are difficult to analyze directly. The models therefore help connect early mammalian development with experimentally accessible cell systems.
Understanding the transition helps researchers control how pluripotent cells respond to differentiation cues and generate specialized cell types. Embryonic stem cell and induced pluripotent stem cell models can therefore support disease research by providing developmentally informed experimental systems. The same knowledge may improve regenerative medicine strategies by making cell-state changes and downstream specialization more deliberate and controllable.