Porcine Subcutaneous Adipose Tissue
Porcine Subcutaneous Adipose Tissue - Related Videos
09:20
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Cited by 47 •
2016
This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.
09:01
A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method
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Cited by 7 •
2017
We standardized an abdominal adipose tissue biopsy using a non-diathermy method performed under local anesthesia. Three cases of excessive post-operation bleeding out of 115 operations (2.61%) occurred.We conclude that an abdominal adipose tissue surgical biopsy using a non-diathermy method can be safely applied to healthy men.
03:28
White and Brown Adipose Depot Collection from Mouse Pup: A Surgical Procedure to Harvest the White Adipose Tissue and Brown Adipose Tissue from Mouse Pup
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2025
In this video, we demonstrate the surgical procedure to isolate the subcutaneous white adipose tissue and interscapular brown adipose tissue from a newborn mouse.
06:35
Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue
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2024
Here, we describe a protocol for isolating, culturing, and phenotyping microvascular endothelial cells from human subcutaneous adipose tissue (hSATMVECs). Additionally, we describe an experimental model of hSATMVEC-adipocyte cross-talk.
08:59
Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique
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Cited by 5 •
2021
The manuscript and associated video demonstrate a percutaneous biopsy technique to obtain samples of subcutaneous adipose tissue from areas surrounding the umbilicus. This method is a low-risk and efficient way to investigate a range of parameters (e.g., gene or protein expression, enzyme activity, lipid content) within adipose tissue.
FAQs
Mesenchymal progenitor cells support tissue development by first proliferating, then committing to the adipocyte lineage, and finally accumulating lipid. These stages connect cell population changes with the formation and expansion of the depot. Examining when progenitors shift between proliferation, commitment, and lipid storage helps developmental biologists relate cellular differentiation to changes in porcine body composition.
Insulin, glucocorticoids, nutrition, and local tissue signals influence adipogenesis, the process by which progenitor cells develop into lipid-storing adipocytes. Their effects help determine how cells progress through lineage commitment and lipid accumulation. Studying these regulatory inputs can reveal how systemic conditions and the local tissue environment jointly shape adipose development during growth.
Expansion reflects both the progression of progenitor cells toward the adipocyte lineage and the subsequent accumulation of lipid within developing adipocytes. This provides a cellular explanation for how a tissue-level change can accompany whole-animal growth. Measuring developmental changes in the depot therefore helps connect cell differentiation and storage capacity with body composition in pigs.
This tissue links cellular development with outcomes that can be observed at the whole-animal level, including growth, body composition, and metabolic regulation. Its study allows researchers to examine how progenitor behavior and adipocyte maturation contribute to those broader traits. The approach is especially relevant when developmental biology seeks connections between tissue formation and organismal physiology.
Research in this depot can address how adipose tissue develops, how obesity-related biology emerges, and how metabolic regulation is influenced during growth. Because the tissue responds to nutritional and hormonal influences, it offers a setting for connecting developmental processes with later physiological outcomes. These questions extend beyond fat storage to the regulation of tissue function and body composition.
Pigs share important anatomical and physiological features with humans, making their subcutaneous adipose tissue useful for investigating adipose development and obesity-related biology. At the same time, developmental changes in the depot can influence body composition and factors related to meat quality. Thus, the model supports both comparative biomedical research and studies of production-relevant traits.