Scalable Production

Scalable production is the systematic expansion of a biological manufacturing process to generate larger quantities of consistent, high-quality material without compromising performance. In developmental biology, it relies on standardized cell sources, controlled culture conditions, reproducible differentiation, and process monitoring to maintain developmental characteristics as production increases. These principles support the generation of cells, tissues, organoids, and other developmental models for research, disease modeling, drug evaluation, and regenerative medicine. Scalable production also improves experimental reproducibility by reducing batch-to-batch variation, while creating a foundation for translating laboratory protocols into reliable, higher-throughput applications.

Scalable Production - Related Videos

Research

JoVE Journal - Developmental Biology
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Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

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Cited by 23 •

2015

The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.

Research

JoVE Journal - Bioengineering

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste

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2025

This protocol presents a scalable method for converting food waste into biodegradable polyhydroxyalkanoate (PHA) bioplastics. It utilizes arrested anaerobic digestion for food waste pretreatment, halophilic microbial fermentation for PHA biosynthesis, and a chemical-free downstream process for PHA recovery.

Research

JoVE Journal - Biochemistry
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Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

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Cited by 4 •

2021

We provide scalable protocols covering construct design, transient transfection, and expression and purification of full-length human huntingtin protein variants in HEK293 cells.

Efficient and Rapid Generation of CAR-T and Cytokine-Induced Killer Cells in GMP-scalable Devices

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2025

This protocol describes a scalable method for the expansion of Adoptive Cell Therapies (ACT) products, namely Chimeric Antigen Receptor (CAR)-T cells and Cytokine-Induced Killer (CIK) cells, using Gas-permeable Rapid expansion (G-Rex) devices, demonstrating efficient and robust growth in a clinically relevant setting.

Research

JoVE Journal - Biology
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Scalable Transfection of Maize Mesophyll Protoplasts

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Cited by 10 •

2023

Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll cells.

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