Three-dimensional Architecture
Three-dimensional Architecture - Related Videos
22:27
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
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Cited by 517 •
2010
The Hi-C method allows unbiased, genome-wide identification of chromatin interactions (1). Hi-C couples proximity ligation and massively parallel sequencing. The resulting data can be used to study genomic architecture at multiple scales: initial results identified features such as chromosome territories, segregation of open and closed chromatin, and chromatin structure at the megabase scale.
12:38
Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
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Cited by 12 •
2017
Here we present an adaptation of the passive CLARITY and 3D reconstruction method for visualization of the ovarian vasculature and follicular capillaries in intact mouse ovaries.
12:07
Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels
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Cited by 4 •
2016
An innovative biofabrication technique was developed to engineer three-dimensional constructs that resemble the architectural features, components, and mechanical properties of in vivo tissue. This technique features a newly developed sacrificial material, BSA rubber, which transfers detailed spatial features, reproducing the in vivo architectures of a wide variety of tissues.
08:33
Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells
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Cited by 22 •
2014
Three dimensional culture of mammary epithelial cells on a reconstituted basement membrane is a useful method to recapitulate the in vivo architecture of the benign breast, and to differentiate the malignant phenotype from the benign breast phenotype. Importantly, this system can be applied to study invasive carcinomas in other tissues.
13:44
Detection of Architectural Distortion in Prior Mammograms via Analysis of Oriented Patterns
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Cited by 13 •
2013
We demonstrate methods for the detection of architectural distortion in prior mammograms. Oriented structures are analyzed using Gabor filters and phase portraits to detect sites of radiating tissue patterns. Each site is characterized and classified using measures to represent spiculating patterns. The methods should assist in the detection of breast cancer.
FAQs
These processes must remain coordinated as development proceeds. Proliferation changes the number of cells, differentiation establishes distinct cell populations, and migration places those populations in new locations. Adhesion helps cells maintain relationships with neighboring cells and extracellular components, while signaling coordinates their behavior. Together, these activities connect cell fate decisions with the progressive shaping of tissues.
Spatial organization links cellular behavior to the form that a developing tissue ultimately acquires. Examining cells only as separate populations can obscure how their positions and interactions contribute to patterning. A three-dimensional perspective allows researchers to relate coordinated cell movements, differentiation, adhesion, and signaling to morphogenesis, the developmental shaping of biological structures.
Extracellular components contribute to the physical context in which cells organize. Their placement relative to cell populations helps establish the structure of a tissue, while cell adhesion connects cells to one another and to their surrounding environment. Studying these relationships can clarify how cellular organization supports tissue form and how altered interactions may influence developmental outcomes.
Two-dimensional cultures provide a simplified setting in which cells grow on a flat surface, whereas three-dimensional models preserve spatial relationships within a physical structure. This distinction can reveal interactions among cells, tissues, and extracellular components that are not captured in planar cultures. Consequently, three-dimensional analysis offers additional context for interpreting morphogenesis and tissue organization.
Researchers can combine three-dimensional models with imaging to examine how cells and extracellular components are arranged and how that arrangement changes over time. Organoids and engineered tissues provide additional systems for studying developmental organization in a structured setting. Comparing observations across these approaches can connect visible architecture with processes such as migration, differentiation, and signaling.
These models are useful when researchers need to investigate tissue organization beyond what two-dimensional cultures can show. They can support studies of morphogenesis, tissue patterning, congenital disorders, regeneration, and tissue engineering by providing structured settings in which cellular interactions and spatial relationships can be examined. Imaging further helps relate those structures to developmental processes and outcomes.