Gel Pores
Gel Pores - Related Videos
06:30
Gel Purification
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2023
Gel purification is used to recover DNA fragments after electrophoretic separation. DNA recovery from an agarose gel includes three basic steps: binding, washing and eluting from a silica column. DNA is believed to bind to silica in the presence of high salt via a salt bridge. Following binding, DNA is washed of impurities and eluted under low salt conditions disrupting this interaction. This video goes through a step-by-step, generalized procedure for cutting out a band from the gel, gel...
01:23
Pore Size Distribution
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2024
In concrete, the pore size distribution significantly influences the material's properties. Capillary pores, markedly larger than gel pores, form a vast network within partially hydrated cement paste, reducing the concrete's strength and increasing its permeability. This heightened permeability leads to a greater risk of damage from environmental factors like freeze-thaw cycles and chemical attacks, with the extent of vulnerability also being tied to the water-to-cement ratio. Adequate...
11:20
Fabrication and Visualization of Capillary Bridges in Slit Pore Geometry
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Cited by 4 •
2014
A procedure for creating and imaging capillary bridges in slit-pore geometry is presented. The creation of capillary bridges relies on the formation of pillars to provide a directional physical and chemical heterogeneity to pin the fluid. Capillary bridges are formed and manipulated using microstages and visualized using a CCD camera.
09:22
DNA Gel Electrophoresis
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2023
DNA gel electrophoresis is a technique used for the detection and separation of DNA molecules. An electric field is applied to a gel matrix comprised of agarose, and within the gel, charge particles will migrate and separate based on size. The negatively charged phosphates of the DNA backbone cause DNA fragments to move toward the anode - a positively charged electrode. The video explains the mechanism by which DNA fragments are resolved on an agarose gel, and it provides a step-by-step...
10:55
Staining Proteins in Gels
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Cited by 7 •
2008
Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. Staining of proteins with Coomassie Blue, Silver Staining, SYPRO Orange, SYPRO Ruby are demonstrated in this video.
FAQs
These processes alter both pore dimensions and the connections between pores. Swelling can increase the space available for liquid, whereas drying can reduce or collapse that space. Cross-linking changes how firmly the network holds its structure, affecting pore size, connectivity, and dimensional stability. Controlling these conditions therefore helps tune transport and mechanical performance.
Pore size describes the scale of individual voids, while connectivity determines whether liquid or gas can move through the network. A material with appropriately sized but poorly connected pores may restrict permeability and diffusion. Evaluating both characteristics gives engineers a more useful basis for predicting water transport, mass movement, and overall material performance.
The pore network interacts with the three-dimensional matrix formed by polymer chains, particles, or reaction products. Changes in void volume and connectivity can influence how the network supports applied loads and maintains its dimensions. Engineering studies therefore consider pore distribution alongside matrix structure when optimizing materials that must balance transport functions with mechanical strength.
A useful workflow begins by controlling the material’s assembly and processing conditions, including solvent retention, swelling, drying, and cross-linking. Researchers then characterize the pore distribution and examine its relationship to permeability, diffusion, water transport, mechanical strength, or dimensional stability. This links internal structure with measurable performance and supports targeted material optimization.
Gel pore analysis applies across several material classes, including hydrogels, cementitious systems, coatings, and porous composites. In each case, the network can influence how fluids move, how dimensions change, and how the material responds mechanically. Comparing pore distributions across these systems helps engineers select processing conditions suited to filtration, sensing, structural, or transport-related requirements.
They are especially important when a material must regulate movement through its internal network. Pore size and connectivity can affect permeability and diffusion in filtration systems, while solvent retention and structural stability matter in tissue engineering and drug delivery materials. Characterizing the distribution helps researchers match internal architecture with the intended transport and performance requirements.