Negative Sequence

Negative sequence is a component of an unbalanced three-phase electrical system in which three equal-magnitude phasors are spaced 120° apart and rotate in the direction opposite to the positive-sequence component. Using symmetrical-component analysis, engineers decompose measured phase voltages or currents into positive-, negative-, and zero-sequence sets, allowing negative-sequence quantities to reveal phase imbalance and asymmetrical faults. Negative-sequence current produces a reverse-rotating magnetic field in generators and motors, which can cause rotor heating, torque pulsations, and reduced equipment life. Engineers therefore use negative-sequence measurements for fault detection, generator and motor protection, power-quality assessment, and diagnosing unbalanced operating conditions.

Negative Sequence - Related Videos

Research

JoVE Journal - Biology

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

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Cited by 13 •

2020

We describe a method to generate saturating transposon mutant libraries in Gram-negative bacteria and subsequent preparation of DNA amplicon libraries for high-throughput sequencing. As an example, we focus on the ESKAPE pathogen, Acinetobacter baumannii, but this protocol is amenable to a wide range of Gram-negative organisms.

Research

JoVE Journal - Biochemistry

Amplicon Sequencing using the Long-Read Sequencing Technologies

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2025

This protocol was optimized for targeted deep sequencing of 18 drug-resistance regions in Mycobacterium tuberculosis using a long-read sequencing platform, followed by analysis with a tuberculosis-specific bioinformatics pipeline designed for long-read data.

Education

JoVE Business - Microeconomics

Negative Externalities

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2025

A negative externality occurs when an economic transaction imposes unintended costs on third parties who are not directly involved in the market transaction. These external costs are not captured in the market price. Negative Externalities and Market Failure Consider a chemical manufacturing plant that produces industrial chemicals for sale. The plant's private costs include raw materials, labor, equipment, and maintenance, which are reflected in the market price of the chemicals. However,...

Positive, Negative, and Zero Work

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2025

Work is done on an object when energy is transferred to the object. In other words, work is done when a force acts on a body that undergoes a displacement from one position to another. By definition, the work done by a force is the integral of the force with respect to the displacement along its path. Forces can vary as a function of position, and displacements can occur along various paths between two points. The magnitude of a force multiplied by the cosine of the angle that the force makes...

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

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Cited by 21 •

2020

This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples.

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