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Biology

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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
 

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Article DOI: 10.3791/61612-v
July 7th, 2020

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Summary July 7th, 2020

We describe a method to generate saturating transposon mutant libraries in Gram-negative bacteria and subsequent preparation of DNA amplicon libraries for high-throughput sequencing. As an example, we focus on the ESKAPE pathogen, Acinetobacter baumannii, but this protocol is amenable to a wide range of Gram-negative organisms.

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