Oversampling

Oversampling is the practice of acquiring or processing a signal at a rate substantially higher than the minimum required to represent its highest frequency component. In engineering, an analog-to-digital converter may sample above the Nyquist rate, then use digital filtering and decimation to reduce bandwidth, suppress aliasing, and improve measurement resolution. The additional samples distribute quantization noise across a wider frequency range, allowing filtering to remove more unwanted noise from the signal band. Oversampling supports accurate audio conversion, sensor instrumentation, communications, and imaging systems, although it increases data volume, processing demands, and power consumption.

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Research

JoVE Journal - Bioengineering
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Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction

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Cited by 10 •

2012

Microscopic organisms like the free-swimming nematode C. elegans, live and behave in a complex three-dimensional environment. We report on a novel approach that provides analysis of C. elegans using diffraction patterns. This approach consists of tracking the temporal periodicity of diffraction patterns generated by directing laser light through a cuvette.

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

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Cited by 12 •

2014

Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton.

Research

JoVE Journal - Biology

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

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Cited by 33 •

2017

Budding yeast is an advantageous model for studying microtubule dynamics in vivo due to its powerful genetics and the simplicity of its microtubule cytoskeleton. The following protocol describes how to transform and culture yeast cells, acquire confocal microscopy images, and quantitatively analyze microtubule dynamics in living yeast cells.

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