High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

The overall goal of this procedure is to measure microtubule dynamics in yeast cells. This method can help answer key questions in the microtubule field, such as how mutations in tubules and microtubule regulators regulate microtubule dynamics in a living cell. The main advantage of this technique is that living yeast cells are stabilized in the image.

Rather, high time-resolution for ten minutes, or low time-resolution for several hours. Demonstrating the procedure will be Colby Fees, and Cassi Estrem, two graduate students from my laboratory. The yeast cells used in this experiment have been constructed to constitutively express GFP labeled tubulin.