Probe-tip Sonication
Probe-tip Sonication - Related Videos
07:24
Sonication Extraction of Lipid Biomarkers from Sediment
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
09:05
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
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2015
Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.
10:10
Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ
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Cited by 5 •
2014
Immunostaining is an effective technique for visualizing specific cell types and proteins within tissues. By utilizing sonication, the protocol described here alleviates the need to dissect Drosophila melanogaster tissues from late-stage embryos and larvae before immunostaining. We provide an efficient methodology for the immunostaining of formaldehyde-fixed whole mount larvae.
03:22
Low Frequency Ultrasound Application: A Method to Determine the Effect of Sonication on Human Leukemia Cells
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2023
This video describes the selective damage of human leukemia cells by using low frequency ultrasound. This application of low frequency ultrasound in a preclinical in vitro condition demonstrates that cell destruction can be attained in order to examine and compare the effects of sonication on leukemia and normal cells.
10:35
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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Cited by 16 •
2017
Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.
FAQs
Alternating pressure cycles create regions of compression and rarefaction in the liquid. When conditions allow cavitation, bubbles form and then collapse rapidly, releasing localized shear, turbulence, and heat. These effects act on suspended particles and droplets, disrupting agglomerates and improving mixing. The resulting processing intensity comes from repeated bubble activity near the vibrating probe.
Amplitude controls the strength of probe vibration and influences how much acoustic energy enters the liquid. Pulse duration determines how long that energy is applied, while treatment volume affects how the delivered energy is distributed. Adjusting these variables helps engineers obtain dispersion or homogenization without excessive heating or unnecessary exposure of sensitive components.
Temperature is both a processing variable and a potential limitation. Cavitation and acoustic energy can raise the liquid temperature during treatment, which may alter the outcome or harm temperature-sensitive components. Monitoring or controlling temperature alongside amplitude and pulse duration allows operators to balance stronger processing against unwanted heating, especially when treating small liquid volumes.
First identify the liquid sample and treatment volume, then position the vibrating metal probe so acoustic energy enters the suspension directly. Set an appropriate amplitude and pulse duration, while accounting for temperature during treatment. The selected conditions should match the objective, such as dispersing nanoparticles, homogenizing a suspension, preparing an emulsion, or processing a sample for materials analysis.
Engineering teams can use the method to disperse nanoparticles, homogenize suspensions, prepare emulsions, and support sample processing for materials analysis. These applications rely on different effects of the treatment, including disrupting particle agglomerates and promoting more uniform mixing. The intended outcome should guide the selected amplitude, pulse duration, temperature, and treatment volume.
The main trade-off is processing intensity versus unwanted effects. Stronger or longer treatment may improve dispersion and mixing, but it can also increase heating and potentially damage sensitive components. Engineers must also consider contamination as a processing risk. Balancing amplitude, pulse duration, temperature, and treatment volume helps preserve sample quality while achieving the target result.