Sample Mounting Procedure
Sample Mounting Procedure - Related Videos
02:57
Kidney Flat Mount Preparation: A Procedure to Dissect and Prepare a Flat Mount for Microscopic Analysis of Adult Zebrafish Kidney
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2023
This video demonstrates the dissection and preparation of a flat mount of the adult zebrafish kidney. The prepared flat mount can be visualized under the microscope for specimen analysis.
08:30
Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation
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Cited by 4 •
2016
Glaucoma is characterized by damage to retinal ganglion cells. Inducing glaucoma in animal models can provide insight into the study of this disease. Here, we outline a procedure that induces loss of RGCs in an in vivo rat model and demonstrates the preparation of whole-mount retinas for analysis.
04:28
Light Sheet Microscopy Sample Preparation: Mounting Live Zebrafish Embryos for Long-Term Imaging
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2023
This article describes sample preparation for light-sheet fluorescence microscopy. It also demonstrates a method to mount live zebrafish embryos for long term imaging, which allows recording of long time-lapse movies of live embryos in near-physiological conditions.
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
04:32
Whole Mount Preparation of Mouse Intestine: Using a Simple Gut-incising Device to Prepare Whole Mount
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2023
In this video, we demonstrate whole mount preparation of the mouse intestine using a simple gut-incising device. The resulting mounts are of superior and uniform quality and can be used for histological analyses.
FAQs
Selection depends on both the specimen material and the intended analysis. Clamps, adhesives, fasteners, and embedding media provide different ways to stabilize a sample, so the choice should support the required examination or evaluation without damaging the specimen or obscuring representative surfaces, dimensions, or interfaces. Matching the approach to these constraints improves reliable interpretation.
Maintaining the intended orientation lets microscopy, mechanical testing, dimensional inspection, or materials characterization examine the relevant surface, dimension, or interface consistently. If positioning changes between specimens, measured differences may reflect mounting rather than the sample itself. Controlled orientation therefore supports representative observations and meaningful comparisons under consistent experimental conditions.
Clamps, adhesives, fasteners, and embedding media are alternative ways to secure a specimen, but the overview does not assign one universally superior role to any of them. The relevant choice is the one that maintains position and orientation while minimizing movement, contamination, or damage. This selection should also preserve the surfaces, dimensions, and interfaces needed for evaluation.
Insufficiently controlled mounting can allow movement, introduce contamination, damage the specimen, or compromise its representative surfaces, dimensions, and interfaces. Those effects can reduce measurement accuracy and make samples less comparable. In engineering work, the mounting condition is therefore part of experimental consistency: observed differences should reflect the specimens or test rather than avoidable variation in support and positioning.
Before analysis, identify the material and the intended evaluation, then select a holder, substrate, or fixture and a compatible securing method. Position and orient the specimen deliberately, establish stable support, and check that mounting has not contaminated or damaged the relevant features. This preparation creates a consistent starting condition for microscopy, testing, inspection, or characterization.
Mounting requirements change with the information sought. Microscopy depends on preserving representative surfaces; mechanical testing requires stable support during evaluation; dimensional inspection depends on maintaining relevant dimensions; and materials characterization may require access to particular surfaces or interfaces. Across these uses, controlled preparation helps maintain measurement accuracy and allows results from multiple samples to be compared consistently.
Repeatable mounting reduces the chance that position, orientation, support, contamination, or damage varies unpredictably from one specimen to another. Consistent conditions make comparisons more defensible because the mounting procedure contributes less uncontrolled variation. This is especially useful when researchers evaluate multiple samples through microscopy, mechanical testing, dimensional inspection, or materials characterization.