Tumor Tissue Handling
Tumor Tissue Handling - Related Videos
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
02:31
FFPE Tumor Tissue Macrodissection: A Technique to Obtain Specific Tumorous Tissue from Unstained Specimens
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2023
This video describes a method for the manual macrodissection of cancer tissue from unstained tissue specimens to obtain a specific cancer tissue. The tissue section can be processed further to obtain DNA for genome-wide studies on gastrointestinal cancers.
09:33
Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis
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2026
This protocol successfully prepared sufficient high-quality neovascular tissue from glioma samples using laser-capture microdissection for quantitative proteomics analysis of tumor angiogenesis, overcoming the limitations of studies that traditionally focus mainly on angiogenic factors and providing a large-scale proteomics profile of glioma angiogenesis.
03:51
Handling Chemical Spills
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2023
Source: Robert M. Rioux & Taslima Zaman, Pennsylvania State University, University Park, PA Since chemicals are commonly used for laboratory research purposes, it is extremely important to be adequately prepared to handle chemical spills or accidental release of hazardous chemicals, which can happen at any time. No matter how minor a spill may be, the inability to respond in an emergency situation could severely endanger public health or the environment. All chemical spills must be properly...
01:28
Pig Handling and Restraint
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Acclimation and Positive Reinforcement Training Pigs are social animals that respond best to calm, low-stress handling techniques. Acclimation and positive reinforcement training are important refinement strategies recommended...
FAQs
Delays after sampling can alter cellular, molecular, and structural features through continued ischemia and degradation. Contamination can introduce additional sources of variation, while poor preservation may disrupt tissue architecture. Controlling these factors helps ensure that later measurements reflect the specimen rather than handling-related damage, which is essential when researchers compare disease mechanisms, therapeutic responses, or engineered tumor systems.
The intended analysis determines how a specimen should be stabilized and prepared. Fixation supports preservation of tissue structure, cryopreservation maintains material for later use, culture enables work with viable tissue, and molecular processing supports analysis of molecular properties. Coordinating the collection and stabilization route with the planned assay reduces the risk of losing features needed for a specific research objective.
Tumor specimens can contain varied cellular and structural features, so inconsistent sampling or processing may change which parts of that variation are represented in an experiment. Standardized handling makes specimens more comparable across studies and helps investigators connect tissue composition and architecture with biological mechanisms or therapeutic response. This consistency is especially important when engineered models are intended to represent patient tumors.
A useful workflow coordinates specimen collection, transport, prompt stabilization, and subsequent processing. Each stage should limit ischemia, degradation, contamination, and unnecessary disruption of tissue architecture. The stabilized material can then be directed toward fixation, cryopreservation, culture, or molecular assays according to the study design. Coordinating these decisions in advance supports reliable downstream analysis and model development.
Carefully handled specimens provide starting material for patient-derived models, engineered tumor microenvironments, biomaterials, and organoid systems. Preserving relevant cellular, molecular, and structural properties allows these platforms to better reflect tumor heterogeneity. As a result, engineering studies can examine disease mechanisms, therapeutic response, or diagnostic and treatment platforms using models that retain more meaningful features of the original tumor.
Consistent processing improves reproducibility and strengthens comparisons between specimens, experiments, and engineered platforms. It helps researchers relate tissue structure and composition to therapeutic response and disease mechanisms while evaluating the performance of diagnostic or treatment systems. In engineering research, reliable handling also supports more credible interpretation of patient-derived models, biomaterials, organoids, and other tumor-mimicking constructs.