Carbon Source Utilization
Carbon Source Utilization - Related Videos
09:30
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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Cited by 14 •
2017
We present an open source high content analysis (HCA) instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts. Data acquisition for this openFLIM-HCA instrument is controlled by software written in µManager and data analysis is undertaken in FLIMfit.
09:19
Utilizing Soil Density Fractionation to Separate Distinct Soil Carbon Pools
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Cited by 3 •
2022
Soil density fractionation separates soil organic matter into distinct pools with differing stabilization mechanisms, chemistries, and turnover times. Sodium polytungstate solutions with specific densities allow the separation of free particulate organic matter and mineral-associated organic matter, resulting in organic matter fractions suitable for describing the soil response to management and climate change.
07:50
Open-source Toolkit: Benchtop Carbon Fiber Microelectrode Array for Nerve Recording
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Cited by 10 •
2021
Here, we describe fabrication methodology for customizable carbon fiber electrode arrays for recording in vivo in nerve and brain.
10:13
Working with Hot and Cold Sources
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2023
Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA Working with extreme temperatures, both high and low, is an integral part of many laboratory operations. For many, mentioning a laboratory instantly evokes the mental picture of a Bunsen burner. Bunsen burners and hot plates are used extensively in small and large operations in research laboratories and industries, thus making it necessary for all users to be aware of their safe handling...
01:14
The Carbon Cycle
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2019
Carbon is the basis of all organic matter on Earth, and is recycled through the ecosystem in two primary processes: one in which carbon is exchanged among living organisms, and one in which carbon is cycled over long periods of time through fossilized organic remains, weathering of rocks, and volcanic activity. Human activities, including increased agricultural practices and the burning of fossil fuels, has greatly affected the balance of the natural carbon cycle. Biological Carbon Cycle All...
FAQs
Transport systems control which carbon compounds can enter a microbial cell, while enzymes determine how efficiently those compounds are processed after uptake. Together, they influence whether a microorganism can use CO2, particular organic substrates, or both. These cellular traits help explain differences in microbial activity across soils, sediments, and aquatic environments.
The route carbon follows depends partly on environmental conditions. Carbon fixation incorporates inorganic carbon into cellular material, whereas respiration and fermentation process available substrates to support metabolism. Because these pathways differ in how carbon is transformed, their relative activity can influence microbial growth, nutrient transformation, and greenhouse-gas fluxes in environmental systems.
Different microorganisms may favor inorganic carbon or distinct organic substrates because their transport systems and enzymes vary. Comparing these preferences provides clues about which groups are active and what metabolic processes they support. This information helps connect community composition with ecosystem functions such as carbon cycling and pollutant degradation.
Investigations can compare microbial responses to inorganic carbon and organic substrates while considering the environmental setting and relevant metabolic pathways. Researchers can then interpret substrate preferences alongside indicators of nutrient transformation, greenhouse-gas flux, or pollutant degradation. This approach links cellular carbon processing with measurable ecosystem activity rather than treating community presence alone as evidence of function.
Carbon-processing patterns help researchers interpret how microbial communities function in different environmental compartments. In soils, sediments, and aquatic systems, the substrates being used and the pathways they enter can indicate ongoing carbon transformations. Such information supports analysis of nutrient cycling, greenhouse-gas production or consumption, and the microbial breakdown of pollutants.
Tracking microbial carbon preferences provides a way to assess active transformations rather than only measuring carbon stocks. Results can help identify changes in greenhouse-gas fluxes, nutrient transformation, or pollutant degradation across environmental systems. These insights contribute to environmental monitoring and can inform strategies designed to understand or manage carbon movement.