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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

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09:30 min

January 18, 2017

DOI:

09:30 min
January 18, 2017

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Summary

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We present an open source high content analysis (HCA) instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts. Data acquisition for this openFLIM-HCA instrument is controlled by software written in µManager and data analysis is undertaken in FLIMfit.

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