Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

This content is Open Access.

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
 

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Article DOI: 10.3791/55119
January 18th, 2017

Chapters

Summary January 18th, 2017

We present an open source high content analysis (HCA) instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts. Data acquisition for this openFLIM-HCA instrument is controlled by software written in µManager and data analysis is undertaken in FLIMfit.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter