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JoVE Journal
Biology

This content is Open Access.

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
 

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Article doi: 10.3791/55119
January 18th, 2017

Summary January 18th, 2017

We present an open source high content analysis (HCA) instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts. Data acquisition for this openFLIM-HCA instrument is controlled by software written in µManager and data analysis is undertaken in FLIMfit.

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