Environmental Chemical Testing
Environmental Chemical Testing - Related Videos
05:44
In vitro Cell Culture Model for Toxic Inhaled Chemical Testing
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Cited by 25 •
2014
This protocol is designed to demonstrate exposure method of cell cultures to inhaled toxic chemicals. Exposure of differentiated air-liquid interface (ALI) cultures of airway epithelial cells provides a unique model of airway exposure to toxic gases such as chlorine. In this manuscript we describe effect of chlorine exposure on air-liquid interface cultures of epithelial cells and submerged culture of cardiomyocytes. In vitro exposure systems allow important mechanistic studies to evaluate...
11:38
Environmentally-controlled Microtensile Testing of Mechanically-adaptive Polymer Nanocomposites for ex vivo Characterization
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Cited by 9 •
2013
A method is discussed by which the in vivo mechanical behavior of stimuli-responsive materials is monitored as a function of time. Samples are tested ex vivo using a microtensile tester with environmental controls to simulate the physiological environment. This work further promotes understanding the in vivo behavior of our material.
07:39
Evaluating the Effect of Environmental Chemicals on Honey Bee Development from the Individual to Colony Level
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Cited by 13 •
2017
Herein we present a method to feed pesticide contaminated food to both an individual honey bee and a beehive colony. The procedure evaluates the pesticide effect on individual honey bees by in vivo feeding of basic larval diet and also on the natural condition of beehive colony.
08:54
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
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Cited by 66 •
2020
Environmental DNA assays require rigorous design, testing, optimization and validation before the collection of field data can begin. Here, we present a protocol to take users through each step of designing a species-specific, probe-based qPCR assay for the detection and quantification of a target species DNA from environmental samples.
10:41
Carbon and Nitrogen Analysis of Environmental Samples
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Elemental Analysis is a method used to determine elemental composition of a material. In environmental samples such as soils, scientists are particularly interested in the amounts of two ecologically important elements, nitrogen and carbon. Elemental analysis by the flash combustion technique works by oxidizing the sample with a catalyst through combustion in a high-temperature chamber. The products of combustion are...
FAQs
Representative sampling helps ensure that test results reflect the environmental material being evaluated rather than an unusual location or isolated portion. Sampling plans therefore consider the relevant medium, such as soil, water, air, or sediment, and the distribution of suspected contaminants. Poorly representative samples can limit the usefulness of otherwise accurate laboratory measurements for monitoring, assessment, or compliance decisions.
Chromatography separates the chemical components of a sample so that individual substances can be examined more clearly. A detector then responds to separated compounds, and the measured response is compared with calibrated standards to estimate their amounts. This combination allows complex environmental samples to be analyzed for contaminants such as pesticides and petroleum compounds rather than treating the sample as a single mixture.
Calibration connects an instrument response to known standards, allowing detector signals to be translated into contaminant measurements. Quality-control procedures then help check whether the analytical process produces accurate and reproducible results. Together, these safeguards strengthen confidence in reported concentrations and help distinguish meaningful environmental findings from problems arising during sample preparation, measurement, or analysis.
The workflow begins with collecting representative samples from the selected environmental medium. Samples are then preserved and prepared before instrumental analysis, which may include chromatographic separation and detector-based measurement against calibrated standards. Quality-control procedures are applied to verify the results. This sequence supports consistent comparison among locations, contaminant types, and sampling periods.
This testing is used when chemical evidence is needed to monitor pollution, assess potential risks, guide remediation, oversee industrial effects, or evaluate regulatory compliance. It can target contaminants in soil, water, air, or sediment, including pesticides, petroleum compounds, and metals. The resulting measurements provide an evidence base for comparing conditions and determining whether environmental quality changes require further attention.
Measurements collected across locations or time periods can show whether contaminant levels remain stable, increase, or decrease. Such comparisons support pollution monitoring and help evaluate the effects of remediation or other environmental changes. Because interpretation depends on reliable sampling, preservation, preparation, calibration, and quality control, consistent procedures are important when tracking trends or judging progress.