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Environment

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JoVE Journal Environment
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
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Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Article DOI: 10.3791/61825-v • 08:54 min • November 5th, 2020
November 5th, 2020 •
*1, *1, *1, *1
1Columbia Environmental Research Center, U.S. Geological Survey
* These authors contributed equally

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Summary
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Environmental DNA assays require rigorous design, testing, optimization and validation before the collection of field data can begin. Here, we present a protocol to take users through each step of designing a species-specific, probe-based qPCR assay for the detection and quantification of a target species DNA from environmental samples.

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Species-specific Quantitative PCR Assays Environmental DNA Applications Optimized Assays Design And Testing Steps Non-invasive Method Conventional Survey Methods Conservation And Wildlife Management DNA Detection Quantitative PCR Sensitivity Contamination Control Negative Controls EDNA Jargon Molecular Biology QPCR Experimental Design Data Generation Gene Regions NCBI's Nucleotide Database
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