Oxygen Flux Monitoring
Oxygen Flux Monitoring - Related Videos
05:47
Oxygen Flux Measurements to Evaluate Mitochondrial Respiration in Living and Permeabilized Cells
0 Views •
2026
Source: Elettra Leo1, Luiza H.D. Cardoso1, Sabine Schmitt1, Erich Gnaiger11Oroboros InstrumentsThis video explains how oxygen flux is used to assess mitochondrial respiration in living and permeabilized mammalian cells. It demonstrates the sequential use of digitonin, metabolic substrates, ADP, an uncoupler, and an inhibitor to characterize different respiratory states. The resulting oxygen flux profile reveals mitochondrial function and energy-coupling efficiency under defined conditions.
03:54
Monitoring B Cell Activation with Antigenic Liposomes using a Calcium-Flux Assay
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2025
This video showcases the assessment of B cell activation through the utilization of antigenic liposomes. B cells treated with calcium-sensitive Indo-1 dye are distinguished using flow cytometry, and their violet-to-blue fluorescence ratio is analyzed. As the cells encounter antigenic liposomes, it triggers B cell activation through calcium influx, progressively enhancing the violet-to-blue fluorescence signal ratio over time.
04:41
Real-Time Monitoring of Energy Metabolism in Human NK Cells Using an Extracellular Flux Analyzer
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2025
This video demonstrates the method to analyze energy metabolism in activated human NK cells using an extracellular flux analyzer. Different inhibitors of the electron transport chain complexes impact ATP synthesis, which is measured through the oxygen consumption rate or OCR, providing insights into cellular metabolism and bioenergetics.
04:39
Extracellular Flux Assay: A Method for Measuring Metabolic Profile of Cells
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2023
This protocol provides a biochemical approach for measuring extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of primary leukemic cells with extracellular flux assay. The assessment of the metabolic profile of these cells helps to characterize their demands, leading to personalized therapy.
11:26
Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer
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Cited by 9 •
2019
The goal of this protocol is to illustrate how to use mouse neonatal cardiomyocytes as a model system to examine how various factors can alter oxygen consumption in the heart.
FAQs
The estimate is derived from the change in oxygen concentration over a measured time interval and is then related to the area through which exchange occurs. This converts a concentration trend into a rate of oxygen production or consumption, allowing measurements from differently sized soils, sediments, or aquatic surfaces to be compared.
Sensors and microelectrodes provide repeated oxygen concentration measurements, while chambers create a defined setting for observing concentration changes associated with a particular environmental surface or sample. Together, these components connect oxygen observations to a known time period and area, supporting quantitative estimates of biological activity and material exchange.
An increase in oxygen over time is consistent with net oxygen production, whereas a decrease indicates net oxygen consumption during the measurement period. Photosynthesis can contribute to production, while respiration and decomposition contribute to consumption. Because several processes may occur together, the measured flux represents their combined effect under the observed conditions.
A concentration change alone does not describe the rate of exchange. Elapsed time determines how quickly oxygen levels changed, and surface area places that change in relation to the size of the measured interface or sample. Including both factors makes the result a flux estimate rather than an isolated concentration observation.
A typical workflow records oxygen concentration with a sensor, microelectrode, or chamber-based setup over a defined period. The observed concentration change is paired with elapsed time and the relevant surface area, then interpreted as oxygen production or consumption. Applying this sequence to soils, sediments, wetlands, or aquatic systems supports comparable environmental measurements.
Researchers apply it when they need evidence about respiration, photosynthesis, decomposition, or sediment-water exchange. The measurements can contribute to assessments of ecosystem metabolism and reveal how oxygen-related activity changes across soils, sediments, wetlands, and aquatic ecosystems. This makes the method useful for connecting local oxygen dynamics with broader ecosystem processes.
Oxygen flux estimates provide a way to examine biological activity and material exchange that are relevant to eutrophication and pollution impacts. Changes in oxygen production or consumption can help characterize altered ecosystem metabolism, while measurements from sediments and water support interpretation of carbon cycling and exchanges occurring at the sediment-water boundary.