Virus-based Microrna Silencing
Virus-based Microrna Silencing - Related Videos
02:59
Potato Virus X-Based MicroRNA Silencing in Potato Plants
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2026
Source: Zhao, J., et al. Potato Virus X-Based microRNA Silencing (VbMS) In Potato. J. Vis. Exp. (2020).This video demonstrates the use of Agrobacterium tumefaciens to deliver a Potato Virus X (PVX)-based construct into in vitro-grown potato plants for virus-based microRNA silencing (VbMS). The method enables systemic suppression of a target microRNA, allowing researchers to study its role in plant development through observable phenotypic changes.
03:42
Evaluating Virus Spread Inhibition Through microRNA Mimic-Mediated Genome Silencing
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2026
Source: Ruiz, A. J. and Russell, S. J. MicroRNA-based Regulation of Picornavirus Tropism. J. Vis. Exp. (2017)This video demonstrates the use of synthetic microRNA mimics to inhibit viral replication in cultured epithelial-like cells selectively. It highlights how microRNA-directed genome silencing can block virus spread and confirm targeting specificity through downstream infectivity analysis.
11:51
Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
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Cited by 8 •
2020
We present a detailed protocol for potato virus X (PVX)-based microRNA silencing (VbMS) system to functionally characterize endogenous microRNAs (miRNAs) in potato. Target mimic (TM) molecules of miRNA of interest are integrated into the PVX vector and transiently expressed in potato to silence the target miRNA or miRNA family.
06:51
Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents
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Cited by 9 •
2018
Here we describe the delivery of microRNA using a recombinant adeno-associated virus serotype 9 in a mouse model of a neuromuscular disease. A single peripheral administration in mice resulted in sustained miRNA overexpression in muscle and motor neurons, providing an opportunity to study miRNA function and therapeutic potential in vivo.
01:22
MicroRNAs
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2019
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
FAQs
The viral construct provides sequences that bind or sequester a selected microRNA, reducing the amount available to regulate its messenger RNA targets. As that repression is diminished, downstream messenger RNAs can escape the microRNA’s post-transcriptional control. Comparing gene regulation with and without silencing helps connect a particular microRNA to cellular or environmental responses.
Sequestration reduces microRNA activity by capturing the molecule with introduced viral sequences, whereas interference with loading acts at the stage when the microRNA would enter the RNA-induced silencing complex. Both routes weaken target regulation, but they affect different points in the silencing process. This distinction can help researchers interpret how a viral construct produces its effects.
The RNA-induced silencing complex is the molecular context in which microRNA-mediated regulation operates. Interfering with microRNA loading into this complex can prevent the microRNA from exerting its usual control over messenger RNAs. Consequently, changes observed after treatment may reflect disrupted microRNA function rather than a direct alteration of the target gene itself.
A study first identifies a microRNA associated with the environmental response of interest, then uses an engineered or naturally occurring viral system containing sequences directed toward that microRNA. The resulting changes in microRNA activity and downstream messenger RNA regulation can be examined under conditions such as drought, salinity, pollutant exposure, or pathogen challenge.
Silencing a candidate microRNA allows researchers to examine whether its normal regulatory activity is associated with responses to drought, salinity, pollutants, or other changing conditions. If reducing that activity alters downstream gene regulation or stress-related behavior, the microRNA becomes linked to the organism’s response. This supports functional genomics and investigation of environmental adaptation.
The method is useful when researchers need to connect a specific microRNA with regulation during pathogen exposure or with traits relevant to environmental stress. By reducing microRNA activity and examining the resulting regulatory changes, studies can identify relationships between small RNAs, host responses, and stress tolerance. These findings can contribute to crop improvement research and broader adaptation studies.