Allelic Exchange
Allelic Exchange - Related Videos
10:35
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
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Cited by 12 •
2020
Described here is a method for targeted, markerless gene deletion in Chlamydia trachomatis using floxed cassette allelic exchange mutagenesis, FLAEM.
02:19
Multiple Allele Traits
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2021
For the same gene multiple alleles can interact to influence phenotypes like the shape and protein composition of an individual cells.By studying allele interactions on the molecular and cellular levels researchers can understand the resulting phenotypes and complications of human conditions like sickle cell trait, improving treatment.The ABO blood group system is a common example of multiple alleles in humans. This system includes three alleles called IA, IB, and i alleles, which combine in...
11:38
Heat Exchanger Analysis
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2023
Source: Alexander S Rattner and Christopher J Greer; Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA Heat exchangers transfer thermal energy between two fluid streams, and are ubiquitous in energy systems. Common applications include car radiators (heat transfer from hot engine coolant to surrounding air), refrigerator evaporators (air inside refrigerator compartment to evaporating refrigerant), and cooling towers in power plants...
02:41
Lethal Alleles
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2021
Agouti: A Lethal Allele Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
09:13
Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
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2024
Chemical genetics involves the substitution of a gatekeeper residue with an amino acid containing a different side chain at the target locus. Here, we have generated a mutant parasite containing a hypomorphic allele of cdpk1 and identified compensatory pathways adopted by the parasite in the mutant background.
FAQs
The donor DNA construct carries sequences matching regions on either side of the chromosomal target. These homologous segments provide the sequence relationships needed for recombination, helping the designed allele align with the corresponding genomic location rather than being incorporated arbitrarily. Their function is central to linking a defined DNA change with a specific genetic locus.
Selectable markers help identify cells that have acquired donor-construct sequences, enriching for candidates after the genetic manipulation. Counterselection can add another screening step by disfavoring cells retaining an unwanted construct or configuration, where the system supports it. Together, these tools make it easier to distinguish cells carrying the intended chromosomal alteration from other genetic outcomes.
Allelic exchange can generate targeted deletions, insertions, point mutations, and sequence corrections. This range allows researchers to remove a gene, add defined DNA, change individual bases, or restore an altered sequence while focusing on one chromosomal locus. Comparing the resulting phenotype with an appropriate reference can help connect that specific sequence change to gene function.
The key distinction is locus specificity. Allelic exchange uses a donor sequence designed around a chosen chromosomal region, so the intended alteration can be connected directly to that site. This supports controlled tests of gene function, whereas approaches that do not target a defined locus provide less direct control over where a sequence change occurs or how it relates to the studied gene.
A typical workflow begins by designing a donor DNA construct containing the desired allele and homologous regions for the target locus. The construct is introduced into a suitable host, and cells associated with the exchange are identified using selectable markers. When available, counterselection helps distinguish the intended replacement from retention of unwanted construct sequences.
The method is useful when researchers need to connect a defined chromosomal change with an observable phenotype. In bacteria and other organisms amenable to homologous recombination, it supports functional genomics, microbial physiology, and studies of genetic regulation. Deletions, insertions, point mutations, and sequence corrections can each provide a focused way to examine gene contribution or regulatory behavior.