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Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

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Medicine

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JoVE Journal Medicine

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

DOI:

10.3791/51742-v

•

08:18 min

•

December 31, 2014

•

Angel Alvarez, Gustavo A. Barisone, Elva Diaz

¹Department of Pharmacology,University of California, Davis

Chapters

00:05Title
01:17Retrovirus Production
03:45NIH 3T3 Cells and Infection
06:02Staining and Foci Quantification
07:05Results: Transcription Factor MXD3 as a Gene of Interest (Potential Oncogene)
07:49Conclusion

Summary

Automatic Translation

The Focus Formation Assay provides a straightforward method to assess the transforming potential of a candidate oncogene.

Tags

Keywords: Focus Formation Assay Oncogenic Potential Ras Transformation NIH 3T3 Cells Retroviral Transduction Cell Transformation Contact Inhibition Anchorage-independent Growth Tumor Formation

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