Rapid Virus Detection
Rapid Virus Detection - Related Videos
05:38
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
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Cited by 4 •
2017
We describe a rapid, molecular-based Influenza A and B assay. The Influenza assay detects each target within 15 min by employing isothermal amplification with influenza-specific primers followed by target detection with molecular beacon probes. The Influenza A and B assay is user-friendly and required minimal hands-on time to perform.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
06:59
Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
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Cited by 7 •
2018
Here, we present a protocol for the rapid virus nucleic acid extraction from the virus-inactivated whole blood. The extraction is performed directly in the blood collection tubes and requires no equipment or electricity. The method is not dependent on laboratory facilities and can be used anywhere (e.g., in field hospitals).
10:05
High-throughput Detection Method for Influenza Virus
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Cited by 8 •
2012
This method describes the use of Infrared dye based imaging system for detection of H1N1 in bronchioalveolar lavage (BAL) fluid of infected mice at a high sensitivity. This methodology can be performed in a 96- or 384-well plate, requires <10 μl volume of test material and has the potential for concurrent screening of multiple pathogens.
09:20
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
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Cited by 16 •
2011
This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.
FAQs
Both approaches increase the amount of a selected viral RNA or DNA sequence so that a detectable signal can be produced. Polymerase-based reactions and isothermal reactions differ in how amplification conditions are maintained, but the source material identifies both as genetic assay formats. Their rapid amplification step helps produce results within minutes to hours for timely research, clinical, or public-health decisions.
Sequence variation can influence whether an assay detects its intended viral target, affecting analytical sensitivity and specificity. A genetic assay depends on target sequences selected during assay design, so changes in viral genomes may alter the match between the assay and circulating variants. Monitoring this relationship is important when interpreting results and when studying viral evolution.
These readouts convert amplified genetic material into an observable result. Fluorescent detection uses a light-associated signal, colorimetric detection produces a visible color change, and probe-based detection relies on a target-associated recognition signal. Because amplification alone is not the final observation, the selected readout determines how researchers or clinicians identify and interpret a positive assay signal.
Antigen formats use antibodies to capture viral antigens rather than measuring viral RNA or DNA. This provides a different type of evidence and can be useful when nucleic-acid testing is unavailable. The two approaches therefore complement one another: genetic assays assess viral material at the sequence level, while antigen formats provide antibody-based evidence of viral components.
A typical workflow begins by extracting viral RNA or DNA, followed by amplification of selected target sequences through a polymerase-based or isothermal reaction. The amplified material is then assessed with a fluorescent, colorimetric, or probe-based readout. Keeping these stages distinct helps connect the final signal to target amplification and supports consistent interpretation across research or testing settings.
The approach is valuable when researchers need timely information about viral genetic material, including during outbreak surveillance and studies of viral evolution. Results can support decisions about infection control and provide data for examining how sequence variation relates to assay sensitivity and specificity. It also connects rapid diagnostic work with broader investigations of changing viral populations.