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Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
DOI:

06:59 min

June 30, 2018

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Chapters

  • 00:04Title
  • 00:44Preparation for Virus Inactivation and Rapid Nucleic Acid Extraction
  • 02:02Blood Collection from Patients with Signs and Symptoms of a Virus Infection
  • 02:50Simplified Nucleic Acid Extraction from Whole Blood
  • 05:04Results: Sensitivity of the Simplified NA Extraction Method
  • 06:22Conclusion

Summary

Automatic Translation

Here, we present a protocol for the rapid virus nucleic acid extraction from the virus-inactivated whole blood. The extraction is performed directly in the blood collection tubes and requires no equipment or electricity. The method is not dependent on laboratory facilities and can be used anywhere (e.g., in field hospitals).

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