Rdna Loci
Rdna Loci - Related Videos
13:19
Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
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Cited by 17 •
2013
Human endogenous retroviruses (HERV), which occupy 8% of the human genome, retain scarce coding capacities but a hundred thousand long terminal repeats (LTRs). A custom Affymetrix microarray was designed to identify individual HERV locus expression and was used on prostate cancer tissues as a proof of concept for future clinical studies.
01:23
Root Loci for Positive-Feedback Systems
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2024
The Hartley oscillator is a positive feedback system that sustains oscillations by feeding the output back to the input in phase, thereby reinforcing the signal. Positive feedback systems can be viewed as negative feedback systems with inverted feedback signals. In these systems, the root locus encompasses all points on the s-plane where the angle of the system transfer function equals 360 degrees. The construction rules for the root locus in positive feedback systems are similar to those in...
06:55
Transfection of a Molecular Clone of Naegleria gruberi rDNA into N. gruberi Trophozoites
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2024
This protocol describes a methodology to transfect Naegleria gruberi trophozoites with a construct that is maintained throughout passaging trophozoites in vitro, as well as through encystment and excystment.
08:03
Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
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2021
This analytical computational platform provides practical guidance for microbiologists, ecologists, and epidemiologists interested in bacterial population genomics. Specifically, the work presented here demonstrated how to perform: i) phylogeny-guided mapping of hierarchical genotypes; ii) frequency-based analysis of genotypes; iii) kinship and clonality analyses; iv) identification of lineage differentiating accessory loci.
09:07
Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci
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Cited by 4 •
2021
Combining DNA halo preparations with fluorescence in situ hybridization enables high-resolution analysis of genomic interactions with the nucleoskeleton. Attached genome leads to hybridized fluorescent signals located within the residual extracted nuclei, whereas non-attached genome is in the halo of DNA surrounding the residual nuclei.
FAQs
RNA polymerase I is the primary enzyme responsible for transcribing the repeated ribosomal RNA genes in eukaryotic rDNA loci. Its activity supplies precursor rRNA for subsequent processing and assembly with ribosomal proteins in the nucleolus. Examining this transcriptional step helps connect locus regulation with ribosome production and with cellular responses to stress or developmental change.
The repeated arrangement of rRNA genes creates a genomic structure in which copy number, recombination, and stability can be studied together. Differences in repeat number can reveal how chromosome organization changes, while recombination within or between repeated units may contribute to genomic instability. These features make rDNA loci useful models for investigating dynamic genome structure in genetics.
Transcription of rRNA genes and early processing of the resulting precursor rRNA occur in association with the nucleolus. Ribosomal proteins then combine with processed rRNA to support ribosome formation. This connection makes the nucleolus a functional context for studying how organization of rDNA loci influences the production and assembly of the molecular machinery required for protein synthesis.
Studies of rDNA loci can assess copy-number variation, recombination, chromosome organization, and genomic instability. These features provide different views of locus behavior: copy number reflects variation among genomic repeats, organization concerns their chromosomal arrangement, and recombination or instability indicates structural change. Together, they help researchers analyze how repeated DNA contributes to genome maintenance and alteration.
Conserved sequences within rDNA loci provide comparable genetic features across organisms or lineages. Researchers can use these shared sequences to examine evolutionary relationships and construct phylogenetic interpretations. Their value comes from allowing sequence-based comparisons while the surrounding locus structure and repeat organization can provide additional context for understanding how genomes have changed over evolutionary time.
Changes in rDNA structure or regulation can serve as indicators of broader cellular and genomic states. In genetics, examining these changes may provide insight into cellular stress responses, developmental processes, and genome alterations associated with disease. The analysis is therefore relevant not only to ribosome biology but also to understanding how genome organization varies across important biological conditions.