Uv-crosslinking Independent
Uv-crosslinking Independent - Related Videos
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
03:40
UV Irradiation: A Method to Integrate Extrachromosomal Arrays
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2023
Microinjection of transgenes usually results in C. elegans strains with large extrachromosomal arrays, which result in variable expression and transmission of the transgene. UV Irradiation can induce chromosomal integration of the array and more stable transgene transmission.
01:21
Introduction to Test of Independence
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2025
In statistics, the term independence means that one can directly obtain the probability of any event involving both variables by multiplying their individual probabilities. Tests of independence are chi-square tests involving the use of a contingency table of observed (data) values. The test statistic for a test of independence is similar to that of a goodness-of-fit test: where: O = observed values E = expected values (which should be at least 5) A test of independence determines whether...
FAQs
The central distinction is whether complexes are maintained through native association or stabilized by a chemical bridge. Native immunoprecipitation attempts to recover RNA-protein or DNA-protein assemblies without creating covalent links. Formaldehyde-based workflows instead fix molecules that are physically close, allowing those relationships to remain associated during purification and later analysis by sequencing.
Formaldehyde acts as a chemical crosslinker that stabilizes molecules located near one another before purification. This is important because associations can otherwise be difficult to retain during sample handling. In genetic and molecular biology experiments, the treatment supports later identification of protein-associated RNA or DNA and helps connect molecular proximity with regulatory interaction maps.
Avoiding ultraviolet exposure reduces dependence on ultraviolet-sensitive interactions and specialized irradiation conditions. This option can suit experiments designed around native immunoprecipitation or chemical stabilization rather than light-induced covalent linking. The decision primarily affects how complexes are preserved before purification, while sequencing remains available as a downstream strategy for identifying associated molecules.
A workflow can begin by preserving complexes either through native immunoprecipitation or chemical crosslinking. The retained material is then purified, and associated nucleic acid or interaction information is examined by sequencing. This sequence of preservation, purification, and readout connects the biochemical treatment to maps of protein binding and molecular associations.
They can help map regulatory protein binding and characterize interactions that influence transcription, RNA processing, translation, and genome regulation. RNA-protein assays are relevant to post-transcriptional processes, whereas DNA-protein analyses can address regulatory associations involving genomic material. Sequencing the purified interaction-associated material provides an experimental route to identifying these relationships.
Native immunoprecipitation is used to recover RNA-protein or DNA-protein complexes through their existing physical associations. Chemical stabilization with formaldehyde instead preserves molecules that are physically close before purification. This distinction gives researchers two preservation strategies: one centered on maintaining native complexes and another designed to retain proximity-based associations during subsequent sample processing.