Uv Light Synthesis
Uv Light Synthesis - Related Videos
05:09
A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria
0 Views •
2025
This video demonstrates the riboflavin and UV light-based pathogen reduction process against high-titer bacterial contamination in human platelet products. This process could help to lower the risk of severe adverse transfusion events associated with bacterial contamination.
10:32
Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
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Cited by 19 •
2015
The storage conditions of platelet products create an ideal environment for bacterial contaminants to multiply to dangerous levels. The goal of this study was to use a colony forming assay to evaluate a riboflavin based pathogen reduction process against high titer bacterial contamination in human platelet products.
08:18
Synthesis and Characterization of High c-axis ZnO Thin Film by Plasma Enhanced Chemical Vapor Deposition System and its UV Photodetector Application
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Cited by 19 •
2015
We offered a method to directly synthesize high c-axis (0002) ZnO thin film by plasma enhanced chemical vapor deposition. The as-synthesized ZnO thin film combined with Pt interdigitated electrode was used as sensing layer for ultraviolet photodetector, showing a high performance through a combination of its good responsivity and reliability.
08:33
Hydrogel Synthesis
0 Views •
2023
Source: Amber N. Barron, Ashlea Patterson, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Hydrogels are a versatile class of cross-linked polymers produced through relatively simple procedures and with generally inexpensive materials. They can be formed from solution and involve a polymer backbone formed from monomer reagents, an initiator which makes the polymer reactive and a crosslinking species which binds the polymer chains...
04:55
UV-Vis Spectroscopy of Dyes - Concepts
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2020
Absorbance When light interacts with a substance, a portion of the light is absorbed while the rest is either reflected or transmitted through it. Substances that we perceive as having a color reflect light in the visible range. The color of the substance that we are able to see depends on which wavelength of light is reflected. A substance that we perceive as blue reflects light in the blue range (430 – 480 nm) of the visible spectrum. According to the color wheel, the same substance absorbs...
FAQs
UV photons activate light-sensitive chemical groups or remove photolabile protecting groups, which temporarily prevents or permits selected reactions. Wavelength, exposure time, and reaction conditions determine when these groups respond and which building blocks can react. Controlling those variables helps restrict synthesis to the intended chemical sites and supports the accurate formation of defined molecular products.
Photolabile protecting groups regulate nucleotide availability during each synthesis cycle. UV exposure removes protection from selected reactive sites, while unexposed sites remain unavailable for coupling. This selective deprotection creates a controlled sequence of chemical reactions, allowing nucleotides to be added in a planned order rather than reacting indiscriminately across the entire support.
Each cycle combines selective deprotection with nucleotide coupling, so the newly available site receives the nucleotide chosen for that step. Repeating this sequence builds DNA incrementally and preserves the intended order of bases. Because the process occurs through controlled cycles, it can generate collections of defined oligonucleotides for downstream genetic assays.
Wavelength and exposure conditions influence whether light-sensitive groups respond and how selectively deprotection occurs. They must be controlled together with the reaction environment to coordinate activation and coupling. Appropriate control supports reproducible synthesis, whereas poorly matched conditions could interfere with the planned sequence of deprotection and nucleotide addition.
The workflow uses a solid support or surface, applies UV radiation to remove selected photolabile protecting groups, and then introduces the nucleotide building block for coupling. Deprotection and coupling are repeated according to the desired sequence. This cycle-based procedure produces spatially organized sets of oligonucleotides rather than a single undifferentiated product.
Spatial addressability allows different oligonucleotide sequences to be produced at defined positions on a surface. Those positions can function as organized probes within a microarray, enabling many molecular interactions to be examined in parallel. In genetics, this arrangement supports gene-expression analysis, mutation detection, and other high-throughput assays.
Light-directed oligonucleotide synthesis can produce probes and microarrays for several genetic applications. The resulting defined DNA materials support gene-expression analysis and mutation detection, while the array format enables high-throughput molecular assays. Its value comes from combining controlled sequence production with the ability to organize distinct probes at addressable locations.