Anti-cd4 Antibody Beads
Anti-cd4 Antibody Beads - Related Videos
06:48
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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Cited by 3 •
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The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.
13:01
Anti-Nuclear Antibody Screening Using HEp-2 Cells
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Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.
06:39
Selective Isolation of CD4+ Vδ1+ γδ T Cells from Human Peripheral Blood Mononuclear Cells
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2025
This video demonstrates a protocol for the magnetic labeling-based isolation of a low-concentration population of CD4+Vδ1+γδ T cells from human peripheral blood mononuclear cells (PBMCs). Using a combination of FITC-conjugated anti-Vδ1 antibodies, magnetic anti-FITC microbeads, and anti-CD4 antibody-coated superparamagnetic beads, the CD4+Vδ1+ T-cells can be successfully purified from the PBMC population.
03:45
A Bead-Based Multiplex Immunoassay to Identify Pathogen-Specific Antibodies in Saliva
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2025
This video demonstrates a bead-based multiplex immunoassay technique to simultaneously detect antibodies for multiple environmental pathogens in human saliva. Fluorescent beads were coupled to antigens from different pathogens, which were captured using antibodies in the human saliva sample. Upon labeling the antibodies using a fluorescent reporter, the beads were analyzed using flow cytometry to assess the presence of different pathogen-specific antibodies in the saliva.
04:57
Detecting Circulating Anti-Glycan Antibodies with a Printed Glycan Array
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2025
This video demonstrates printed glycan array technology for analyzing circulating anti-glycan antibodies in mouse serum. The antibodies in the serum interact with specific glycans on the chip, which is confirmed by immunostaining followed by fluorescence scanning.
FAQs
Selectivity comes from antigen-specific recognition: the immobilized antibody binds the CD4 surface protein, so particles associate preferentially with cells displaying that marker. In a mixed sample, this interaction helps distinguish CD4-expressing cells from cells lacking the target. The resulting bead-cell association provides the basis for controlled separation or concentration before downstream analysis.
Magnetic formats provide a physical recovery mechanism in addition to antibody-mediated recognition. After the beads associate with CD4-expressing cells, an external magnetic field can help collect the bead-cell complexes or remove them from the sample. This supports rapid enrichment or depletion and makes the selected cell population accessible for subsequent immunology or infection studies.
The intended outcome depends on which fraction is retained after separation. Recovering bead-associated cells enriches the sample for CD4-expressing cells, whereas removing those complexes depletes that population from the original mixture. These opposite uses allow investigators to compare samples with increased or reduced CD4+ cell representation while examining immune responses or cellular behavior.
A basic workflow brings the antibody-coated particles into contact with the mixed cell population so CD4 recognition can occur. The bead-associated fraction is then separated or concentrated, using an external field when the particles are magnetic. Researchers can recover the selected cells, remove them from the sample, or prepare the resulting population for downstream analysis.
They are useful when researchers need to examine CD4+ cells within a heterogeneous population. Applications include enriching or removing these cells to investigate host responses to pathogens, adaptive immune mechanisms, and the cellular context of infection. Selection can also help focus experiments on CD4+ T-cell function, activation, or susceptibility to infection.
The separated population can support downstream examination of how CD4+ cells function, become activated, or respond during infection-related studies. Comparing enriched, depleted, or concentrated fractions helps connect CD4-cell representation with host responses and adaptive immune mechanisms. The approach therefore serves as a preparation step for analyzing cellular behavior rather than as an endpoint by itself.