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JoVE Journal
Biology

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Anti-Nuclear Antibody Screening Using HEp-2 Cells
 

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Article DOI: 10.3791/51211
June 23rd, 2014

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Summary June 23rd, 2014

Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.

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