Bone Marrow Culture
Bone Marrow Culture - Related Videos
06:39
Culture of myeloid dendritic cells from bone marrow precursors
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Cited by 22 •
2008
This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.
04:00
Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia
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2025
In this video, we demonstrate the isolation and culture of bone marrow stromal cells from the long bones of mice. The isolated adherent BMSCs can be used for further analysis.
03:56
Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology
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2023
This video describes the technique of aspirating bone marrow from iliac spine and smear preparation for biopsy. In the example, we perform bone marrow aspiration from an acute myeloid leukemia (AML) patient and examine the cells to check for measurable residual disease (MRD), an important prognostic biomarker.
08:15
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
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Cited by 13 •
2009
This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.
07:06
Bone Marrow-derived Macrophage Production
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Cited by 205 •
2013
Macrophages have long been recognized as a critical component of the innate and adaptive immune responses. The recent explosion of knowledge pertaining to evolutionary, genetic, and biochemical aspects of the interaction between macrophages and microbes has renewed scientific attention to macrophages. This article describes a method to differentiate macrophages from mouse bone marrow.
FAQs
Growth factors help determine which marrow-derived cells survive, expand, or differentiate during culture. Their inclusion in nutrient media supports controlled investigation of hematopoietic and immune-cell development rather than treating the aspirate as a uniform population. By examining how cell populations change under defined conditions, researchers can study factors that influence blood formation and immune function.
Defined laboratory conditions help preserve the experimental context in which marrow cells are studied. Nutrient media, appropriate growth factors, and controlled incubation support cell maintenance while allowing researchers to observe expansion, differentiation, or cellular responses. Consistency in these conditions makes it easier to relate observed changes to the treatment or microbial stimulus being investigated.
Marrow-derived immune cells can be maintained with microbial stimuli or in systems designed to reveal infectious agents. Researchers then examine how immune-cell populations respond and how host-pathogen interactions develop under ex vivo conditions. This approach connects cellular behavior with infection-related mechanisms, helping characterize immune responses without relying only on observations from an intact organism.
The workflow begins with processing a bone marrow aspirate to obtain cells for laboratory use. Researchers place the cells in nutrient media containing appropriate growth factors, then maintain them under defined incubation conditions. Subsequent observation focuses on whether populations survive, expand, differentiate, respond to microbial stimuli, or reveal infectious agents relevant to the experiment.
Changes in cultured populations can indicate how hematopoietic or immune cells develop under selected conditions. Survival may show that the culture supports maintenance, whereas expansion or differentiation can provide evidence of altered cellular development. Responses to microbial stimuli or detection of infectious agents add information about immune activity and host-pathogen interactions.
This culture system is useful when researchers need to examine immune-cell development, blood formation, or cellular responses to microbial stimuli in a controlled ex vivo setting. It also supports studies of bone marrow disorders and treatments that affect hematopoiesis or immune function. Its applications therefore span both basic mechanisms and evaluation of disease- or treatment-related effects.