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JoVE Journal Biology
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Culture of myeloid dendritic cells from bone marrow precursors

Article DOI: 10.3791/769
July 25th, 2008 •
1,2, 2,3, 2, 2
1Medical Sciences Program, McMaster University, 2Centre for Gene Therapeutics, McMaster University, 3Department of Chemical Engineering, University of Waterloo

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July 25th, 2008

This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.

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