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Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
 

Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins

Article doi: 10.3791/54786
November 18th, 2016

Summary November 18th, 2016

Novel isolation methods of primary endothelial cells from blood vessels are needed. This protocol describes a new technique that completely inverts blood vessels of interest, exposing only the endothelial side to enzymatic digestion. The resulting pure endothelial cell culture can be used to study cardiovascular diseases, disease modelling, and angiogenesis.

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