Journal
/
Medicine
/
Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins

Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins

DOI:
10.3791/54786-v

•

08:24 min

•

November 18, 2016

•

, , ,
1Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine,Utrecht University

Chapters

  • 00:05Title
  • 00:53Isolation and Culture of Primary Canine Endothelial Cells
  • 04:30Characterization of Canine Aortic Endothelial Cells
  • 06:14Results: Canine Primary Endothelial Cell Isolation and Culture
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

Novel isolation methods of primary endothelial cells from blood vessels are needed. This protocol describes a new technique that completely inverts blood vessels of interest, exposing only the endothelial side to enzymatic digestion. The resulting pure endothelial cell culture can be used to study cardiovascular diseases, disease modelling, and angiogenesis.

Tags

Keywords: Primary Endothelial CellsCanine ArteriesCanine VeinsCardiovascular ResearchAngiogenesisTumor AngiogenesisAtherosclerosisCell IsolationCell CultureGelatinHBSSCollagenaseDispaseCECGMCentrifugation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54786?article_type=v

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
check_url/54786?article_type=v

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
check_url/54786?article_type=v

Primary Culture of Human Vestibular Schwannomas
check_url/54786?article_type=v

Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens
check_url/54786?article_type=v

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
check_url/54786?article_type=v

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
check_url/54786?article_type=v

Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model
check_url/54786?article_type=v

Isolation and Culture Expansion of Tumor-specific Endothelial Cells
check_url/54786?article_type=v

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
check_url/54786?article_type=v

A Cell Culture Model of Resistance Arteries

Read Article