Cord Blood Cd34+ Cells
Cord Blood Cd34+ Cells - Related Videos
05:23
Isolating Cord Blood Hematopoietic Stem Cells to Generate Human Immune System Mice
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2025
This video demonstrates a technique for generating human immune system (HIS) mice. CD34+ hematopoietic stem cells (HSCs) are isolated from human cord blood buffy coat cells using antibody-coated magnetic beads. The CD34+ HSCs are expanded in a growth medium enriched with cytokines and cryopreserved. Finally, the thawed HSCs are injected into an immunodeficient mouse pup to establish the HIS mouse model.
09:46
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
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Cited by 21 •
2017
A highly pure population of megakaryocytes can be obtained from cord blood-derived CD34+ cells. A method for CD34+ cell isolation and megakaryocyte differentiation is described here.
10:25
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
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Cited by 7 •
2019
Here, we present a protocol for immunophenotypic characterization and cytokine induced differentiation of cord blood derived CD34+ hematopoietic stem and progenitor cells to the four myeloid lineages. The applications of this protocol include investigations on the effect of myeloid disease mutations or small molecules on myeloid differentiation of the CD34+ cells.
10:42
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid
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Cited by 9 •
2019
Here, we describe the ex vivo expansion of hematopoietic stem cells from CD34+ cells derived from umbilical cord blood treated with a combination of a cytokine cocktail and VPA. This method leads to a significant degree of ex vivo expansion of primitive HSCs for either clinical or laboratory applications.
14:06
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
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Cited by 2 •
2013
Here we describe a protocol for isolating subsets of precursor B-cells from umbilical cord blood. A sufficient quantity and quality of nucleic acids may be extracted from the cells and used in subsequent assays utilizing DNA or RNA.
FAQs
The CD34 surface marker provides a practical way to identify the target hematopoietic stem and progenitor population within cord blood. In experimental workflows, investigators can use that defining feature to focus analyses on cells with blood-forming potential rather than treating the entire cord-blood sample as equivalent. This improves the biological specificity of studies of hematopoiesis and immune development.
Self-renewal allows these cells to maintain a stem and progenitor population, while differentiation produces increasingly specialized blood and immune cells. Together, these properties connect an initial progenitor population with the later appearance of lymphoid and myeloid lineages. Studying both processes helps researchers examine how blood formation and immune-cell development are established.
Lymphoid and myeloid differentiation represents two major routes through which blood-forming progenitors contribute to immune development. Examining these outcomes enables researchers to investigate how distinct immune-cell lineages arise from cord blood CD34+ cells. This is particularly relevant when studying the cellular basis of host responses to pathogens, because infection research can involve development of more than one immune lineage.
These cells provide a system for examining how blood-forming progenitors contribute to the restoration of immune-cell populations. Researchers can evaluate factors that influence immune reconstitution by following the relationship between progenitor potential and the development of lymphoid and myeloid cells. The resulting information connects hematopoietic activity with the recovery or establishment of immune function.
Collection begins with umbilical cord blood obtained after birth, followed by identification of cells expressing the CD34 surface marker. The resulting population can then serve as the focus of hematopoiesis, immune-development, or transplantation research. This collection source is useful because it provides access to cells with developmental potential before they have fully differentiated into mature blood and immune lineages.
Investigators can use cord blood CD34+ cells to model the generation of immune cells that participate in host responses to pathogens. The approach links progenitor development with later immune biology, allowing studies to examine how the capacity to produce lymphoid and myeloid cells relates to infection-related responses. It therefore supports research on immune development and pathogen response in the same experimental context.
Their ability to self-renew and produce blood and immune lineages makes cord blood CD34+ cells relevant to hematopoietic stem cell transplantation and cellular-therapy research. These applications depend on developmental potential rather than on a single mature immune function. Research can therefore assess how progenitor properties may support blood formation, immune reconstitution, or therapeutic cell development.