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Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

DOI:
10.3791/50402-v

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14:06 min

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April 16, 2013

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, , , ,
1Department of Pathology and Anatomical Sciences,University of Missouri-Columbia, 2Laboratory for Infectious Disease Research,University of Missouri-Columbia

Chapters

  • 00:05Title
  • 02:12Isolation of Mononuclear Cells from Umbilical Cord Blood
  • 04:19Modified B-cell Isolation from Mononuclear Cells Using MACS Separation
  • 06:32Antibody Labeling and Preparation for Cell Sorting
  • 08:32Cell Sorting Using the MoFlo XDP Flow Cytometer
  • 11:07Results: Isolation of Cord Blood Pre-cursor B-cell Subsets
  • 13:13Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol for isolating subsets of precursor B-cells from umbilical cord blood. A sufficient quantity and quality of nucleic acids may be extracted from the cells and used in subsequent assays utilizing DNA or RNA.

Tags

Keywords: Umbilical Cord BloodHematopoietic Progenitor CellsPrecursor B-cellsB-cell DifferentiationCell IsolationCell SortingCell Surface MarkersMononuclear CellsBiotinFluorescent LabelingDownstream Nucleic Acid Assays

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