Cytosolic Calcium
Cytosolic Calcium - Related Videos
05:10
Imaging Calcium Dynamics in Pancreatic Cells: A Technique to Study Real-time Changes in Cytosolic Calcium Concentration in Pancreatic Islet
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2023
This video describes the technique of imaging islet cells in real-time, to study the changes in cytosolic calcium concentration in the minor subpopulations of the pancreatic islet cells. Minor cell subpopulations can be identified using functional responses to marker compounds, such as adrenaline and ghrelin, that induce changes in cytosolic calcium in a specific populations of islet cells. This can provide an insight into the cross talk between the blood glucose and the islet secretory output.
09:33
Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
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Cited by 2 •
2020
Brain capillary pericytes are essential players in the regulation of blood-brain barrier properties and blood flow. This protocol describes how brain capillary pericytes can be isolated, cultured, characterized with respect to cell type and applied for investigations of intracellular calcium signaling with fluorescent probes.
08:08
Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
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Cited by 24 •
2011
We introduce an approach to evaluate the cytosolic Ca2+ concentration in isolated lymphatics to study Ca2+-dependent and Ca2+-sensitizing mechanisms of lymphatic smooth muscle contraction.
10:24
Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
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Cited by 8 •
2014
Calcium is involved in numerous physiological and pathophysiological signaling pathways. Live cell imaging requires specialized equipment and can be time consuming. A quick, simple method using a flow cytometer to determine relative changes in cytosolic calcium in adherent epithelial cells brought into suspension was optimized.
13:55
Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
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Cited by 40 •
2013
Vacuolar and cytosolic pH can be measured in live yeast (S. cerevisiae) cells using ratiometric fluorescent dyes localized to specific cellular compartments. We describe procedures for measuring vacuolar pH with BCECF-AM, which localizes to the vacuole in yeast, and cytosolic pH with a cytosolic ratiometric pH-sensitive GFP (yeast pHluorin).
FAQs
Calcium signals are generated by two coordinated sources: calcium can enter through plasma-membrane channels, or it can be released from intracellular stores, particularly the endoplasmic reticulum. Their contribution determines how an external signal is converted into a cytoplasmic change. This distinction helps investigators ask whether a response reflects membrane entry, store release, or both.
Pumps and exchangers restore cytosolic calcium toward its baseline after a signal occurs. This recovery prevents the elevated concentration from persisting indefinitely and allows the cell to respond to later stimulation. In immune cells, effective restoration is therefore important for maintaining controlled calcium-dependent signaling rather than allowing activation-related changes to continue without regulation.
Changes in cytosolic calcium regulate calcium-dependent enzymes and transcription factors, which connect an incoming signal to immune-cell behavior. Through these downstream targets, calcium signaling influences lymphocyte activation, cytokine production, phagocytosis, and degranulation. Measuring the signal can therefore help relate a change in intracellular calcium to a specific functional immune response.
Infectious agents can disrupt the cellular communication controlled by calcium signaling. Such disruption may affect how external signals are converted into immune-cell responses, including lymphocyte activation, cytokine production, phagocytosis, or degranulation. Examining calcium changes during host-pathogen interactions helps researchers investigate whether altered signaling accompanies impaired or modified immune-cell function.
Cytosolic calcium measurements reveal changes in intracellular signaling as immune cells respond to external stimuli or infectious conditions. Researchers can use these measurements to investigate lymphocyte activation, cytokine production, phagocytosis, and degranulation, while also examining how calcium dynamics relate to host-pathogen interactions and broader inflammatory signaling.
The overview identifies immune-cell calcium signals as transient and shows that pumps and exchangers return cytosolic levels toward baseline. Recording both the change and its recovery can therefore connect signaling onset with subsequent regulation. This information helps researchers evaluate how calcium-dependent pathways participate in immune activation and how infection may alter cellular communication.