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Biology

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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
 

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Article DOI: 10.3791/51857
October 28th, 2014

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Summary October 28th, 2014

Calcium is involved in numerous physiological and pathophysiological signaling pathways. Live cell imaging requires specialized equipment and can be time consuming. A quick, simple method using a flow cytometer to determine relative changes in cytosolic calcium in adherent epithelial cells brought into suspension was optimized.

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