Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Cytosolic Calcium Measurements in Renal…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
Ex Vivo Method for Assessing the Mouse Reproductive Tract Spontaneous Motility and a MATLAB-based Uterus Motion Tracking Algorithm for Data Analysis
Journal (Biology)
Ex Vivo Method for Assessing the Mouse Reproductive Tract Spontaneous Motility and a MATLAB-based Uterus Motion Tracking Algorithm for Data Analysis
Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches
Journal (Biology)
Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches
 

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Article DOI: 10.3791/51857 • 10:24 min • October 28th, 2014
October 28th, 2014 •
1, 2
1Institute for Physiology, Pathophysiology, & Toxicology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke, 2Institute for Immunology & Experimental Oncology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke

Chapters

Summary
Automatic Translation

Calcium is involved in numerous physiological and pathophysiological signaling pathways. Live cell imaging requires specialized equipment and can be time consuming. A quick, simple method using a flow cytometer to determine relative changes in cytosolic calcium in adherent epithelial cells brought into suspension was optimized.

Tags

Cytosolic Calcium Measurements Renal Epithelial Cells Flow Cytometry Cellular Processes Calcium Signaling Calcium Binding Dyes Live Cell Imaging Fluorescence Intensity Emission Wavelength Alternative Detection Methods Immunological Cells Non-immunological Adherent Cells Fluorescence Microplate Reader Epithelial Cells Renal Proximal Tubule Cells Cell Permeable Calcium Binding Dye
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter