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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
JoVE Journal
Biology
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JoVE Journal Biology
Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

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10:24 min

October 28, 2014

DOI:

10:24 min
October 28, 2014

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Summary

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Calcium is involved in numerous physiological and pathophysiological signaling pathways. Live cell imaging requires specialized equipment and can be time consuming. A quick, simple method using a flow cytometer to determine relative changes in cytosolic calcium in adherent epithelial cells brought into suspension was optimized.

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