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10:24 min
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October 28, 2014
DOI:
Summary
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Calcium is involved in numerous physiological and pathophysiological signaling pathways. Live cell imaging requires specialized equipment and can be time consuming. A quick, simple method using a flow cytometer to determine relative changes in cytosolic calcium in adherent epithelial cells brought into suspension was optimized.
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Cite this Article
Lee, W., Dittmar, T. Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry. J. Vis. Exp. (92), e51857, doi:10.3791/51857 (2014).
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