Dorsal Collection
Dorsal Collection - Related Videos
09:48
Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration
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Cited by 9 •
2017
This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration.
04:38
Modeling Sensory Axon Regeneration in a Rat Using Dorsal Root Ganglion Injection and Dorsal Root Crush Injury
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2025
Source: Cheah, M., et al. Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration. J. Vis. Exp. (2017).This video demonstrates a step-by-step procedure for injecting a viral suspension into the dorsal root ganglia (DRGs) and inducing a controlled axonal injury via a dorsal root crush in an anesthetized rat. The protocol involves exposing the DRGs, delivering a growth-promoting viral vector to facilitate axonal regeneration, and severing axonal...
06:39
Live Imaging of Dorsal Root Axons after Rhizotomy
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Cited by 6 •
2011
An in vivo imaging protocol to monitor primary sensory axons following dorsal root crush is described. The procedures utilize wide-field fluorescence microscopy and thy1-YFP transgenic mice, and permit repeated imaging of axon regeneration over 4 cm in the PNS and axon interactions with the interface of the CNS.
03:43
Neuronal Enrichment of Dorsal Root Ganglia by Immunopanning
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2025
This video demonstrates a protocol for enriching neuronal cultures from dorsal root ganglia by immunopanning, thereby improving their purity and utility for neurological research.
06:56
Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation
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2025
This video demonstrates an enzyme-free protocol to isolate macrophages from mouse dorsal root ganglion (DRG) using a mechanical dissociation technique. Upon homogenization of the freshly collected DRG tissue, antibodies against the cell-surface receptors on the macrophages are used to label the cells fluorescently, followed by performing flow cytometry to confirm the presence of macrophages in the sample.
FAQs
Consistency in subject positioning, dorsal-site access, sample transfer, and handling helps ensure that differences between specimens reflect biological variation rather than sampling conditions. Standardized collection also limits avoidable contamination and tissue damage. In infection and immunology studies, this improves reproducibility and supports meaningful comparisons among experimental groups, time points, or treatment conditions.
Sterile instruments and careful transfer reduce the chance that external contaminants will enter the specimen or interfere with laboratory analysis. Limiting tissue damage is also important because unnecessary disruption may affect the material being examined. Together, these precautions help preserve sample quality and strengthen confidence in measurements of pathogens, immune mediators, or local host responses.
Researchers should keep the subject’s positioning, access to the dorsal surface, instrument handling, and sample-transfer conditions as consistent as possible. These factors can influence contamination risk, tissue damage, and the comparability of collected material. Applying the same conditions across experimental groups makes changes in infection, inflammation, or treatment response easier to interpret.
A typical workflow begins by positioning the subject to provide controlled access to the dorsal surface. The area is then accessed with sterile instruments, and the tissue, fluid, or other specimen is collected and transferred for laboratory analysis. Throughout the procedure, researchers aim to limit contamination and tissue damage while maintaining consistent conditions between samples.
Material obtained through this approach can support pathogen detection, measurement of immune mediators, and characterization of local host responses. These readouts allow researchers to examine how infection-related changes appear in the sampled region. When collections are performed consistently, the resulting data can also help compare disease progression or treatment effects across experimental groups.
This approach is useful when investigators need to assess local biological responses during infection, inflammation, or treatment. Repeatedly applying comparable sampling conditions can support evaluations of progression over time and differences between experimental groups. The collected material therefore contributes to studies that connect local host responses with pathogen-related findings and therapeutic outcomes.