Dual Marker Detection
Dual Marker Detection - Related Videos
08:45
Duplex Digital PCR for Simultaneous Quantification of Dual Genetic Markers
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2025
In this video, we demonstrate the duplex digital PCR (ddPCR) technique — a modification of the traditional PCR technique that is useful in detecting two different genetic markers simultaneously. A single PCR reaction is partitioned into nanoliter-sized emulsified droplets that are independently amplified, and the detection of differently colored fluorescence amplification signals from the fraction of droplets is used to compute the initial concentration of the target sequences.
02:40
Detecting SMAD Pathway Activation Using Dual Adenovirus Transduction
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2026
Source: Chen, H., et al. Live Cell Imaging of the TGF-β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System. J. Vis. Exp. (2018).This video demonstrates a method to study SMAD pathway activation in breast cancer cells using dual adenoviral infection. One virus delivers a constitutively expressed GFP gene, while the other carries an RFP reporter under a SMAD-sensitive promoter. Upon TGF-beta stimulation, SMAD proteins are activated, forming a complex that induces RFP...
03:16
Dual Fluorescence Imaging to Identify Differentiated Oligodendrocytes
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2025
Source: Schott, J. T., et al. Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning. J. Vis. Exp. (2016). This video demonstrates the use of dual fluorescence imaging to evaluate the differentiation of oligodendrocytes from precursor cells (OPCs) isolated from a rat pup brain. The process includes fixing the cells, labeling specific markers with primary and fluorophore-conjugated secondary antibodies, and...
08:45
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
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Cited by 22 •
2017
This study implemented whole genome sequencing for analysis of mutations in genes conferring antifungal drug resistance in Candida glabrata. C. glabrata isolates resistant to echinocandins, azoles and 5-flucytosine, were sequenced to illustrate the methodology. Susceptibility profiles of the isolates correlated with presence or absence of specific mutation patterns in genes.
11:35
Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
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Cited by 16 •
2010
A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.
FAQs
Each marker-specific antibody or probe is linked to a signal that can be differentiated from the signal assigned to the other marker, such as a separate fluorescence channel. The assay can therefore record both readouts during the same analysis and determine whether each marker is present independently or detected together, improving resolution within a mixed biological sample.
Co-localization indicates that the two selected signals occur in the same cellular or sample context, rather than merely appearing somewhere in the specimen. This is useful when the research question depends on marker combinations, such as identifying cells that carry both an immune-cell feature and an activation-associated feature. The result supports more specific cellular characterization.
A single marker may identify a broad population or state, while the combination of two markers narrows the interpretation by showing which cells or sample regions meet both criteria. In immunology, this can separate immune-cell subsets or distinguish activation states more precisely. The paired result reduces reliance on an isolated signal when biological categories overlap.
The workflow begins by selecting marker-specific antibodies or probes for the two targets and assigning distinguishable signals to them. Both detection reagents are applied to the same sample, after which the signals are measured simultaneously. Analysis then compares each marker's presence and evaluates whether the signals are co-localized, producing a combined rather than separate readout.
It is especially useful when researchers need to define immune-cell subsets or assess cellular activation and functional states. Pairing markers allows a population to be described by a combination of characteristics instead of one feature alone. This can improve interpretation of immune responses by linking cell identity with a relevant state in the same experimental readout.
Examining a host marker together with a pathogen marker can help distinguish infected cells from uninfected cells while also identifying the relevant cellular context. The combined pattern supports analysis of how infection relates to immune-cell characteristics or activation. In infection research, these results can contribute to studying disease mechanisms and evaluating experimental or diagnostic outcomes.