Hollow Fiber Filtration
Hollow Fiber Filtration - Related Videos
08:28
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
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Cited by 28 •
2016
The functional behavior of cells in culture can be improved by culturing in more in vivo-like 3-dimensional culture environments16-21. This manuscript describes the set-up and operation of a hollow fiber bioreactor system for in vivo-like mammalian tissue culture.
12:11
A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
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Cited by 11 •
2012
This protocol describes the use of a tangential flow hollow-fiber ultrafiltration sample concentration system and a heat dissociation as alternative steps for the detection of waterborne Cryptosporidium and Giardia species using EPA Method 1623.
06:01
Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture
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Cited by 2 •
2019
The goal of this protocol is production of whole extracellular matrix fibers targeted for wound repair which are suitable for preclinical evaluation as part of a regenerative scaffold implant. These fibers are produced by the culture of fibroblasts on hollow fiber membranes and extracted by dissolution of the membranes.
01:32
Filtration and Urine Formation
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2019
The function of the kidneys is to filter, reabsorb, secrete, and excrete. Every day the kidneys filter nearly 180 liters of blood, initially removing water and solutes but ultimately returning nearly all filtrates into circulation with the help of osmoregulatory hormones. This process removes wastes and toxins but is also crucial to maintain water and electrolyte levels. Most of these functions are performed by the tiny but numerous nephrons contained within the kidneys. Blood enters the renal...
01:26
Glomerular Filtration: Net Filtration Pressure
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2025
Glomerular filtration, a key process in the kidneys, is regulated by three main pressures: Glomerular blood hydrostatic pressure (GBHP), Capsular hydrostatic pressure (CHP), and Blood colloid osmotic pressure (BCOP). GBHP, with an average value of 55 mmHg, promotes filtration by pushing water and solutes through the filtration membrane. This is balanced by two opposing forces: CHP, a "back pressure" exerted against the filtration membrane by fluid already in the capsular space and renal tubule,...
FAQs
Selective retention depends on the relationship between the membrane’s pores and the size or properties of material in the fluid. Components that cannot pass through remain in the retentate, while smaller or otherwise permeable components enter the filtered stream. Adjusting these characteristics allows researchers to target cells, microorganisms, particles, or macromolecules for different experimental purposes.
Pressure provides the driving force for movement across the fiber membranes, but flow conditions influence how efficiently components separate. Changes in these conditions can alter which materials pass through and which remain concentrated. Controlling them is therefore important when the goal is clarification, concentration, washing, or recovery of biologically valuable material.
The retentate is the portion held back by the semipermeable fibers, whereas the filtered fluid contains components that cross the membrane. This distinction helps researchers direct cells, pathogens, particles, or macromolecules into the fraction most useful for subsequent analysis. Tracking both fractions can clarify whether a target was retained, removed, or collected in the filtrate.
Its relatively gentle operation can support recovery of biological material without relying on harsh separation conditions described in the source material. This is valuable when samples must remain suitable for downstream culture, immunoassays, molecular analysis, or bioprocessing. The approach therefore combines selective processing with a practical emphasis on maintaining material for later study.
A sample may be clarified by removing unwanted material, concentrated by retaining selected components, or washed by separating them from the surrounding fluid. The same membrane-based workflow can support pathogen or immune-cell separation from complex biological fluids. Researchers select the intended goal according to which fraction they need for downstream work.
The method is useful when investigators need to prepare complex biological samples for host-pathogen studies or immune analysis. It can separate or concentrate pathogens and immune cells before culture, immunoassays, or molecular analysis. Its scalability also makes it relevant to bioprocessing, where controlled handling of biological fluids is required beyond a small analytical sample.