Leukocyte Population Enumeration
Leukocyte Population Enumeration - Related Videos
14:45
Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
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Cited by 14 •
2012
In this report, we demonstrate the staining and analysis steps of a phenotyping assay performed on fresh whole blood to enumerate major innate and adaptive leukocyte populations. We emphasize considerations for performing these procedures in the context of a multicenter clinical trial.
09:29
Algae Enumeration via Culturable Methodology
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Algae are a highly heterogeneous group of microorganisms that have one common trait, namely the possession of photosynthetic pigments. In the environment, algae can cause problems for swimming pool owners by growing in the water. Algae can also cause problems in surface waters, such as lakes and reservoirs, due to algal blooms that release toxins. More recently, algae...
10:53
Serial Dilutions and Plating: Microbial Enumeration
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2023
Source: Jonathan F. Blaize1, Elizabeth Suter1, and Christopher P. Corbo1 1 Department of Biological Sciences, Wagner College, 1 Campus Road, Staten Island NY, 10301 Quantitative assessment of prokaryotes can be onerous given their abundance, propensity for exponential proliferation, species diversity within a population, and specific physiological needs. Compounding this challenge, is the four-phase nature in which bacteria replicate (lag, log, stationary and death). The ability to accurately...
10:57
Culturing and Enumerating Bacteria from Soil Samples
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Authors: Bradley Schmitz and Luisa Ikner Surface soils are a heterogeneous mixture of inorganic and organic particles that combine together to form secondary aggregates. Within and between the aggregates are voids or pores that visually contain both air and water. These conditions create an ideal ecosystem for bacteria, so all soils contain vast populations of bacteria, usually over 1 million...
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
2011
A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
FAQs
Enumeration depends on separating leukocyte populations using observable or lineage-associated features. Cell size and morphology provide physical and visual cues, whereas surface markers connect a cell to a particular lineage. Combining these signals helps distinguish populations within the same sample and makes the resulting immune-cell profile more informative than a single total white-cell measurement.
Absolute counts and population proportions answer different questions. A population may represent a smaller percentage because another leukocyte group expanded, even if its own number remained stable; conversely, its percentage can remain similar while total leukocyte abundance changes. Reporting both measures therefore supports more careful comparisons of immune status across experimental conditions.
Changes in leukocyte composition can be examined alongside infection-related conditions to characterize how host defense is being altered. The value lies not only in detecting that the overall leukocyte profile changed, but in identifying which populations contributed to that change. This population-level view helps connect immune-cell composition with inflammatory responses during infection.
Morphology and cell size may reveal that populations differ, but they do not necessarily identify lineage. Lineage-associated surface markers add a biologically linked criterion for assigning cells to populations. This distinction matters when different leukocyte groups share broad physical features, because marker-based classification can produce a more specific profile for comparing immune responses.
An analysis proceeds by examining a biological sample, assigning leukocytes to populations according to selected measurable features, and calculating each population's number or proportion. The selected criteria should match the comparison being made, such as a focus on morphology or lineage. The resulting values can then be compared across experimental conditions to identify composition changes.
Interpretation should distinguish a shift in one population from a broad change affecting the whole leukocyte profile. Numbers show abundance, while proportions show representation within the measured population. Reviewing both helps determine whether an apparent increase reflects expansion of that group, contraction of others, or a more general alteration in immune-cell composition.
It is useful when researchers need to compare immune status between experimental conditions, examine infection-associated changes, or characterize an inflammatory response. The same measurement can also reveal whether a treatment changes leukocyte composition. Because it resolves the profile into populations rather than relying on a single aggregate value, it supports more targeted interpretation of host-defense changes.
Treatment-related changes can be assessed by comparing the numbers or proportions of leukocyte populations across treated and comparison conditions. A changed profile may indicate that treatment altered immune-cell composition, even when the overall immune response is considered broadly. In infection research, this comparison helps evaluate how an intervention relates to inflammatory or host-defense patterns.