Lung-on-chip
Lung-on-chip - Related Videos
08:35
Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip
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Cited by 12 •
2015
Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.
01:35
Organ-on-Chip Models
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2026
Introduction to Organ-on-Chip Models Organ-on-chip models are advanced microphysiological systems that recreate key structural and functional features of human organs in a controlled laboratory setting. These devices integrate living human cells within microfluidic channels, allowing researchers to simulate tissue–tissue interfaces, fluid flow, and mechanical forces that are essential for normal organ function. Unlike traditional cell cultures, which often lack these dynamic physiological cues,...
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
06:10
Accessible Silicone Chip-to-Membrane Sealing Procedure for Flexible, Reliable Bonding
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2026
A novel sealing protocol to produce water-tight silicone-based seals for membranes of Polydimethylsiloxane (PDMS) chip devices. Intended for labs setting-up chip models and small-scale chip platform development. We demonstrate the protocol using plastic and native tissue membranes. This procedure only requires PDMS, toluene, mold, membrane, and a vacuum chamber.
FAQs
The permeable membrane keeps lung and vascular cells in separate but closely interacting channels. This arrangement preserves the organization of the alveolar-capillary interface while allowing relevant molecular signals and cellular effects to be observed across the barrier. Its design helps investigators examine how infection or inflammation alters communication between the lung-facing and blood-facing compartments.
Fluid perfusion and cyclic mechanical stretch reproduce two important physical conditions of the lung: blood flow and breathing-related motion. Including these cues places cultured cells in a more physiologically relevant environment than static culture alone. Because the conditions are controlled, investigators can observe how respiratory mechanics influence barrier behavior, inflammatory signaling, or responses to infection.
These models can follow several connected stages of respiratory infection, including pathogen entry, epithelial barrier disruption, and inflammatory signaling. Real-time observation links visible changes at the tissue interface with responses developing in adjacent compartments. That continuity helps researchers study infection as a dynamic interaction between the pathogen, lung tissue, vascular cells, and surrounding immune processes.
Lung-on-chip systems provide a controlled setting for examining how immune cells interact with infected or stressed lung and vascular tissues. Investigators can relate those cellular interactions to barrier damage and inflammatory signaling while monitoring events at the alveolar-capillary interface. This makes the approach especially relevant to immunology and infection studies focused on host responses rather than pathogens alone.
A typical workflow establishes lung and vascular cells in adjacent microfluidic channels separated by a permeable membrane, then maintains the system with fluid perfusion and cyclic stretch. Researchers can study pathogen entry or add immune-related conditions while observing barrier integrity, signaling, and cell interactions. The controlled format supports direct comparison of different experimental treatments or conditions.
Researchers may use these models to evaluate host-directed therapies, antimicrobial treatments, or vaccine responses in a human lung-like experimental context. The system can reveal effects on tissue barriers, inflammatory signaling, and interactions among lung, vascular, and immune components. It also offers a way to investigate respiratory biology while reducing reliance on animal studies.