Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

Hsien-San Hou; Hui-Fang Chang; Ji-Yen Cheng

doi:10.3791/53340

Journal

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Bioengineering

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Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

8,600 Views

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08:35 min

•

December 29, 2015

DOI:

10.3791/53340-v

DOI:

10.3791/53340-v

•

08:35 min

December 29, 2015

•

3 Views

Hsien-San Hou¹, Hui-Fang Chang¹, Ji-Yen Cheng^1,2,3,4,5

¹Research Center for Applied Sciences,Academia Sinica, ²Institute of Biophotonics,National Yang-Ming University, ³Biophotonics & Molecular Imaging Research Center (BMIRC),National Yang-Ming University, ⁴Department of Mechanical and Mechatronic Engineering,National Taiwan Ocean University, ⁵Ph.D. Program in Microbial Genomics,National Chung Hsing University

Summary

Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.