Lymph Gland Dissection
Lymph Gland Dissection - Related Videos
04:20
Larval Lymph Gland Dissection: Isolating the Drosophila Hemocyte-Producing Organ
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2023
This video describes the Drosophila lymph gland—the primary site of hematopoiesis in the fly larva—and shows an example protocol to dissect and mount the organ for immunohistochemistry and fluorescent imaging.
11:49
Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
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Cited by 5 •
2016
Drosophila and mammalian hematopoietic systems share many common features, making Drosophila an attractive genetic model to study hematopoiesis. Here we demonstrate dissection and mounting of the major larval hematopoietic organ for immunohistochemistry. We also describe methods to assay various larval hematopoietic compartments including circulating hemocytes and sessile crystal cells.
03:31
Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes
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Cited by 58 •
2007
The mosquito midgut and salivary glands are key entry and exit points for vector pathogens like Plasmodium falciparum and the dengue virus. This video demonstrates the dissection techniques for removing the midgut and salivary glands from Aedes aegypti mosquitoes.
16:48
Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
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Cited by 20 •
2007
Two-photon imaging has uncovered lymphocyte motility and cellular interactions within the lymph node under basal conditions and durring an immune response 1. Here, we demonstrate adoptive transfer of T cells, isolation of lymph nodes, and imaging motility of CD4+ T cells in the explanted lymph node.
04:07
Larval Ring Gland Dissection: Isolation of Developmental Endocrine Organs from Drosophila
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2023
This video describes the Drosophila ring gland, a complex endocrine structure important for biosynthesis and secretion of ecdysteroids and other hormones. The featured protocol demonstrates its dissection, fixation, and immunostaining.
FAQs
Maintaining these anatomical regions preserves the spatial organization needed to interpret immune activity. The capsule, cortex, and medulla can be examined as distinct tissue compartments, while intact lymphatic connections help retain information about tissue organization and trafficking. This structural context strengthens histological assessment and helps relate cellular findings to localized immune responses.
Lymph node tissue contains information about antigen presentation, lymphocyte activation, and clonal expansion. Examining resident and recruited immune cells allows investigators to assess how an immune challenge changes cellular composition and organization. Comparing these findings across tissues or experimental conditions can clarify how localized responses contribute to broader systemic immunity.
Their preserved tissue architecture and lymphatic connections provide a basis for examining how immune cells enter, move through, and accumulate within lymphoid tissue. Dissection enables researchers to analyze these features through histology, cell isolation, or flow cytometry. The resulting data can connect tissue organization with recruitment during infection, inflammation, or other immune challenges.
A typical workflow isolates the lymph node from surrounding tissues while minimizing disruption to recognizable anatomical regions and lymphatic connections. The recovered tissue is then directed toward an appropriate analysis, such as histology, immune-cell isolation, flow cytometry, molecular assays, or pathogen studies. The selected downstream method determines how the specimen should be preserved and processed.
Dissected lymph nodes can support several complementary analyses. Histology examines tissue organization, whereas cell isolation and flow cytometry characterize resident or recruited immune populations. Molecular assays investigate biological changes in the tissue, and pathogen studies assess infection-related findings. Using more than one approach can connect cellular composition, structure, and molecular responses.
In this research context, dissected lymph nodes provide a localized view of responses to infection, vaccination, inflammation, and other immune challenges. Investigators can examine antigen presentation, lymphocyte activation, clonal expansion, and immune-cell trafficking within the same tissue system. These observations help relate events in lymphoid organs to localized and systemic immune outcomes.