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JoVE Journal
Biology

This content is Open Access.

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
 

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

Article DOI: 10.3791/265
August 23rd, 2007

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Summary August 23rd, 2007

Two-photon imaging has uncovered lymphocyte motility and cellular interactions within the lymph node under basal conditions and durring an immune response 1. Here, we demonstrate adoptive transfer of T cells, isolation of lymph nodes, and imaging motility of CD4+ T cells in the explanted lymph node.

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