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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

Article DOI: 10.3791/265 • 16:48 min • August 23rd, 2007
August 23rd, 2007 •
1, 2, 1
1Department of Physiology and Biophysics, University of California, Irvine (UCI), 2Department of Neurobiology and Behaviour, University of California, Irvine (UCI)

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Summary
Automatic Translation

Two-photon imaging has uncovered lymphocyte motility and cellular interactions within the lymph node under basal conditions and durring an immune response 1. Here, we demonstrate adoptive transfer of T cells, isolation of lymph nodes, and imaging motility of CD4+ T cells in the explanted lymph node.

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Dissection 2-Photon Imaging Peripheral Lymph Nodes Mice Adoptive Transfer Labeled T Cells Isolation Imaging Motility CD4+ T Cells Explanted Lymph Node Two-photon Imaging Of Immune Cells Fluorescently Labeled Cells Cell Tracker Dye Fluorescent Protein Tail Vein Injection Lymphoid Organs Isolation Of Lymph Node Excised Lymph Node Oxygenation Of Perfused Media Temperature Laser Power 3D Video Images Naive CD4+ T Cells
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