Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

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Biology

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Biology

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JoVE Journal Biology

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

DOI:

10.3791/2645-v

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09:38 min

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May 31, 2011

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Rita Nokes Cook, Su Fen Ang*¹, Richard Seung-on Kang*¹, Heike Fölsch

¹Department of Cell and Molecular Biology,Northwestern University

Chapters

00:05Title
01:27Preparation of DNA and MDCK Cells
02:04Microinjection Procedure and Post-injection Incubations
06:07Surface Staining for Immunofluorescence Analysis
08:21VSVGts045-GFP Control in MDCK Cells
09:05Conclusion

Summary

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This article details the procedures involved in overexpression and analysis of small GTPases in polarized epithelial cells using microinjection technique.

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

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