Microfluidic Paper Device
Microfluidic Paper Device - Related Videos
07:53
Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices
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Cited by 4 •
2016
We demonstrate the use of patterned aerosol adhesives to construct 3D paper microfluidic devices. This method of adhesive application forms semi-permanent bonds between layers, enabling single-use devices to be non-destructively disassembled after use and to ease folding complex nonplanar structures.
11:33
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Cited by 12 •
2017
We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.
08:29
Thermal Measurement Techniques in Analytical Microfluidic Devices
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Cited by 6 •
2015
Here, we present three protocols for thermal measurements in microfluidic devices.
10:18
Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
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Cited by 26 •
2017
Multilayer microfluidic devices often involve the fabrication of master molds with complex geometries for functionality. This article presents a complete protocol for multi-step photolithography with valves and variable height features tunable to any application. As a demonstration, we fabricate a microfluidic droplet generator capable of producing hydrogel beads.
29:02
Cell Capture Using a Microfluidic Device
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Cited by 1 •
2007
FAQs
Capillary action draws a liquid sample through hydrophilic paper channels, allowing movement without an external pump. The patterned layout directs the sample toward regions containing reagents or capture zones, where processing and detection occur. This passive flow mechanism reduces equipment requirements and supports compact testing formats suitable for settings where powered fluid-handling systems are unavailable.
Embedded reagents interact with components in the sample, while capture zones retain specific target molecules, cells, or pathogens. These localized reactions generate measurable signals when the relevant target is present. Separating fluid movement, chemical processing, and signal generation across patterned regions helps the device convert a small sample into an interpretable analytical result.
Using small amounts of sample and reagent can reduce material requirements and support compact device designs. This feature is especially relevant when blood, saliva, or other specimens are limited or difficult to collect. Combined with passive fluid transport and simple operation, low-volume analysis can contribute to more accessible testing outside conventional laboratory settings.
In this research context, the platform can support detection of antigens, antibodies, and other biomarkers associated with immune responses or infection. Blood and saliva are examples of compatible sample types identified for these applications. The specific target determines which embedded reagent or capture zone is needed to produce a measurable signal.
A typical workflow begins by applying a small liquid sample to the patterned paper. Capillary action then carries it through hydrophilic channels toward embedded reagents and capture zones. Interactions with targets in the sample generate a measurable signal, which can then be used to determine whether the relevant antigen, antibody, biomarker, cell, or pathogen is present.
They are useful when testing must be rapid, portable, and operationally simple, including point-of-care settings and field-based disease surveillance. In immunology and infection research, they can help assess antigens, antibodies, and other biomarkers using samples such as blood or saliva. Their low resource requirements may improve access to testing where conventional laboratory infrastructure is limited.