Microwell Seeding
Microwell Seeding - Related Videos
02:25
Photodegradable Hydrogel-Based Isolation of Bacterial Colonies in Microwell Arrays
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2025
Source: Fattahi, N., et al. Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation. J. Vis. Exp. (2021)This video demonstrates the use of photodegradable hydrogels to isolate bacterial colonies within silicon microwell arrays. Bacterial cells are first loaded into microwells, encapsulated in a crosslinked hydrogel, and cultured into distinct colonies. Upon exposure to ultraviolet light, targeted degradation of the hydrogel enables selective extraction of individual...
06:28
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
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Cited by 11 •
2016
Conventional methods to initiate suspension aggregate based cardiac differentiation of human pluripotent stems cells (hPSCs) are plagued with culture heterogeneity with respect to aggregate size and shape. Here, we describe a robust method for cardiac differentiation employing microwells to generate size-controlled hPSC aggregates cultured under cardiac-promoting conditions.
10:44
Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates
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Cited by 36 •
2013
We introduce a protocol for the generation of large numbers (thousands to hundreds of thousands) of uniform size- and composition-controlled tumor spheroids, using commercially available microwell plates.
02:33
Seed Structure and Early Development of the Sporophyte
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2020
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo. The embryo contains a double set of chromosomes, one set from each parent. Fertilization of the haploid egg by the haploid sperm gives rise to the zygote, which develops into the embryo. The endosperm is a...
08:52
A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
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Cited by 21 •
2013
We present the procedure to assemble a seed coat bedding assay (SCBA) from Arabidopsis thaliana seeds. The SCBA was shown to be a powerful tool to explore genetically and in vitro how the endosperm controls seed germination in dormant seeds and in response to light cues. The SCBA is in principle applicable under any situation where the endosperm is suspected to influence embryonic growth.
FAQs
Well geometry and seeding density regulate how often cells contact one another, how strongly they aggregate, and how consistently they form clusters. Because the wells confine cells at a microscale, changing either variable can alter cluster organization and introduce differences between culture units. Controlling both is therefore central to obtaining comparable cellular responses.
Gravity-driven settling places cells within the confined well space, creating defined microscale environments for local cellular interactions. This organization makes it easier to standardize how immune, host, or microbial cells occupy each culture unit. As a result, researchers can examine cluster formation and cell-cell interactions with less variation between wells.
Using defined cell numbers makes each microscale culture a more controlled experimental unit. It helps limit variation in starting conditions, allowing differences in aggregation, cellular interaction, infection response, immune activation, or treatment effect to be interpreted against a more standardized baseline. This consistency is especially useful when comparing multiple wells or experimental conditions.
A basic workflow begins by distributing a defined number of cells into regularly arranged small wells, then allowing them to settle by gravity in the confined spaces. Researchers can culture the resulting microscale units and use them for microscopy, phenotyping, or functional assays. Consistent seeding and well conditions support comparable results across experimental units.
Microwell cultures can be examined by microscopy, phenotyping, and functional assays. These readouts can characterize cellular interactions, infection responses, immune activation, and treatment effects within standardized culture units. The format is therefore useful both for observing how cells organize into clusters and for assessing functional changes associated with infection, activation, or intervention.
In immunology and infection research, the approach supports reproducible co-cultures that bring immune, host, and microbial systems into controlled microscale contact. This organization helps researchers study interactions and responses while reducing variation between experimental units. It can also provide a consistent platform for comparing infection conditions, immune activation states, or treatment effects.