Recombinant Fibronectin
Recombinant Fibronectin - Related Videos
01:25
Fibronectins Connect Cells with ECM
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2023
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal. Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 7 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
08:09
Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay
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Cited by 5 •
2024
This protocol details the isolation of chondrocytes, Fibronectin Adhesion Assay-derived Chondroprogenitors (FAA-CPs), and Migratory Chondroprogenitors (MCPs) from human articular cartilage. It covers enzymatic digestion, fibronectin adhesion, and migration-based assays for isolating and characterizing these cells.
08:56
Recombineering and Gene Targeting
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2023
One of the most widely used tools in modern biology is molecular cloning with restriction enzymes, which create compatible ends between DNA fragments that allow them to be joined together. However, this technique has certain restrictions that limit its applicability for large or complex DNA construct generation. A newer technique that addresses some of these shortcomings is recombineering, which modifies DNA using homologous recombination (HR), the exchange between different DNA molecules based...
02:31
Homologous Recombination
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2020
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
FAQs
Recombinant fibronectin presents several binding regions rather than a single interaction site. These regions can engage integrins on cells as well as collagen, proteoglycans, and other extracellular components. Their combined activity gives researchers a way to examine how specific matrix contacts organize adhesion and signaling, including the conditions that support immune-cell attachment or movement.
Integrin contacts connect the extracellular matrix environment with immune-cell behavior. In a defined fibronectin-based system, researchers can assess whether matrix attachment is associated with changes in leukocyte adhesion, migration, or activation. This helps separate effects arising from matrix contact from broader culture conditions and provides a focused framework for investigating inflammation at tissue interfaces.
Matrix remodeling is relevant because changes in extracellular contacts can alter the context in which inflammation and microbial adhesion occur. Studying these interactions with recombinant fibronectin allows investigators to relate matrix organization to leukocyte responses and to pathogen binding at host surfaces. The approach therefore connects extracellular-matrix biology with infection-model design.
Researchers can incorporate Recombinant Fibronectin in two principal formats described for this topic: as a defined cell-culture substrate or in a binding assay. The substrate format supports controlled examination of cell attachment, migration, and activation, while binding assays help characterize interactions involving extracellular components, immune cells, or pathogens.
Binding assays can help characterize how pathogens interact with host-surface components in a controlled experimental setting. When recombinant fibronectin is included, the assay can focus attention on matrix-associated contacts rather than treating the host surface as an undefined background. Results can inform studies of microbial adhesion and the construction of infection models.
It provides a defined extracellular-matrix context for examining cell behavior relevant to tissue repair and for developing biomaterials. In immunology and infection studies, this context can also be used to investigate how matrix contacts influence leukocyte responses or pathogen interactions. These applications help link material design and repair processes with inflammatory and infectious outcomes.