Mouse Primary Antibodies
Mouse Primary Antibodies - Related Videos
05:31
A Multiplex Immunostaining Method Using Primary Antibodies from the Same Host Species
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2025
This video demonstrates an assay to perform multiplex immunostaining using primary antibodies from the same host species. In this method, an adrenal tissue section undergoes a two-step immunostaining process. Initially, the section is stained with antibodies specific for markers in the cortex, followed by stripping the bound antibody and restaining with antibodies specific for markers in the medulla.
03:18
Antibody-Dependent Enhancement of Zika Virus by Dengue Antibodies in Human Primary Macrophages
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2026
Source: Asad, S., et al. Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells. J. Vis. Exp. (2019)This video demonstrates antibody-dependent enhancement of Zika virus infection in human primary macrophages, using dengue antibody–virus complexes to model how cross-reactive antibodies facilitate Fcγ receptor–mediated viral entry and replication for studying infection mechanisms and immune interactions.
11:58
Primary Culture of Mouse Dopaminergic Neurons
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Cited by 66 •
2014
Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.
02:51
An Ultrasound-Assisted Delivery of Antibodies in a Mouse Model
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2025
This video demonstrates a procedure of ultrasound-assisted antibody delivery in a mouse model. Retro-orbitally injected microbubbles oscillate under ultrasound, creating a transient disruption in the blood-brain barrier, permitting labeled antibodies to enter the brain.
11:27
Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
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Cited by 2 •
2008
We describe a reliable method for immunolocalization within the epidermis modified for both frozen and paraffin sections that we use very routinely in our laboratory.
FAQs
Recognition depends on the variable regions, whose shapes and chemical properties complement specific antigenic epitopes. These regions bind targets through noncovalent interactions, so the match between antibody and epitope determines which protein, pathogen component, or immune marker produces a detectable signal. Specificity is therefore central to distinguishing the intended molecular target from unrelated material.
Isotype is an important selection characteristic because it can affect how signals are interpreted and how consistently experiments perform. The antibody constant region also provides the recognition site for labeled secondary antibodies, allowing the primary antibody-target interaction to become measurable. Considering both properties helps researchers choose reagents that support reliable detection or functional assay designs.
Specificity determines whether an observed signal reflects the intended antigen rather than unrelated material. Experimental controls provide a comparison for judging that signal and help reveal whether detection is dependable under the chosen assay conditions. Together with deliberate isotype selection, these considerations improve interpretation, reduce ambiguity, and support reproducibility across immunology and infection experiments.
The assay should reflect whether the goal is localization, measurement, or characterization of protein expression. Immunofluorescence and immunohistochemistry are suited to localizing targets, whereas enzyme-linked immunosorbent assays support measurement. Western blotting helps characterize changes in protein expression. Selecting the format this way connects the antibody signal to the biological question being asked.
These reagents can reveal immune markers and changes in host-response proteins, while also identifying microbial components in experimental samples. Their use across localization, measurement, and protein-expression assays allows investigators to examine where relevant targets occur and how their levels change. This makes them useful for connecting molecular detection with host-pathogen responses.
A labeled secondary antibody recognizes the constant region of the mouse primary antibody after the primary antibody has bound its target. This creates a detectable signal without requiring the antigen-binding reagent itself to carry the label. The approach supports signal generation in assays such as immunofluorescence, immunohistochemistry, enzyme-linked immunosorbent assays, and western blotting.